Department of Bacteriology and California Primate Research Center, University of California, 95616, Davis, California, USA.
Curr Microbiol. 1978;1(3):157-62. doi: 10.1007/BF02601669.
During a 4-year period 43 cases of spontaneous lymphoma occurred in macaques at the California Primate Research Center. In an attempt to determine if there might be a common viral etiology to the outbreak, lymphoma tissues from 10 rhesus monkeys were explanted into tissue culture and examined for the presence of virus. Electron microscopic studies of the cultured lymphoma cells revealed viruses morphologically characteristics of adenovirus, reovirus, foamy virus, and herpesvirus. Cell-free filtrates from tissue cultures possessing the latter three viruses produced cytopathology in virus-free indicator cells. Virus particles typical of the explant culture were demonstrable in the infected indicator cells. Type-C RNA tumor virus particles were not observed electron microscopically in any of the lymphoma tissues or lymphoma cell cultures examined.
在 4 年期间，加利福尼亚灵长类动物研究中心的猕猴发生了 43 例自发性淋巴瘤。为了确定爆发是否可能存在共同的病毒病因，将 10 只恒河猴的淋巴瘤组织进行体外培养，并检查病毒的存在。对培养的淋巴瘤细胞的电子显微镜研究显示，病毒在形态上具有腺病毒、呼肠孤病毒、泡沫病毒和疱疹病毒的特征。具有后三种病毒的组织培养物的无细胞滤液在无病毒指示细胞中产生细胞病变。在受感染的指示细胞中可检测到与体外培养物相似的病毒颗粒。在检查的任何淋巴瘤组织或淋巴瘤细胞培养物中均未观察到电子显微镜下的 C 型 RNA 肿瘤病毒颗粒。
