细粒棘球绦虫复合种G1和G6基因型鉴别中的幼虫钩长度测量
Larval hook length measurement for differentiating G1 and G6 genotypes of Echinococcus granulosus sensu lato.
作者信息
Harandi Majid Fasihi, Hajialilo Elham, Shokouhi Mostafa
机构信息
Department of Parasitology, Kerman University of Medical Sciences, Kerman, Iran.
出版信息
Turkiye Parazitol Derg. 2012;36(4):215-8. doi: 10.5152/tpd.2012.52.
OBJECTIVE
Echinococcus granulosus is a globally important cestode parasite causing remarkable medical and economical losses in the world. Ten genotypes (G1-G10) have been identified within this complex species. Protoscoleces rostellar hook characters e.g. total large and small hook lengths may be useful to differentiate genotypes. This study investigates the value of rostellar hook morphometry on genetically identified isolates of E. granulosus using mitochondrial cox1 and nad1 sequencing.
METHODS
In total, 24 hydatid cyst samples of livestock and human origin were collected. The isolates were then sequenced for the mitochondrial cox1 and nad1 genes and total large and small rostellar hook lengths of protoscoleces were measured.
RESULTS
Total large and small hook lengths could differentiate between G1 and G6 genotypes; however, G1 and G3 were not distinguishable by hook morphometry. Only large hook length was significantly different between the G3 and G6 isolates.
CONCLUSION
The G6 genotype is readily distinguishable from G1 by using both small and large hook lengths; however, only total large hook length was significantly different between the G3 and G6 genotypes.
目的
细粒棘球绦虫是一种在全球具有重要意义的绦虫寄生虫，在世界范围内造成了显著的医学和经济损失。在这个复合物种中已鉴定出10种基因型（G1 - G10）。原头节顶突钩的特征，如大、小钩的总长度，可能有助于区分基因型。本研究利用线粒体细胞色素c氧化酶亚基1（cox1）和烟酰胺腺嘌呤二核苷酸脱氢酶亚基1（nad1）测序，探讨顶突钩形态测量在基因鉴定的细粒棘球绦虫分离株中的价值。
方法
总共收集了24份家畜和人类来源的包虫囊肿样本。然后对分离株的线粒体cox1和nad1基因进行测序，并测量原头节大、小顶突钩的总长度。
结果
大、小钩的总长度可以区分G1和G6基因型；然而，通过钩形态测量无法区分G1和G3。G3和G6分离株之间只有大钩长度存在显著差异。
结论
通过使用小钩和大钩长度，G6基因型很容易与G1区分开来；然而，G3和G6基因型之间只有大钩总长度存在显著差异。
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