基于氧化石墨烯的表面增强拉曼散射探针用于癌细胞成像。

Graphene oxide based surface-enhanced Raman scattering probes for cancer cell imaging.

机构信息

MOE Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.

出版信息

Phys Chem Chem Phys. 2013 Feb 28;15(8):2961-6. doi: 10.1039/c2cp43715e. Epub 2013 Jan 22.

DOI:10.1039/c2cp43715e

PMID:23340832

Abstract

The intrinsic Raman signals provide the potential of graphene oxide (GO) for cellular imaging. Herein, novel surface-enhanced Raman scattering (SERS) labels based on GO-Ag nanoparticle (NP) composites are developed for fast cellular probing and imaging. The optimum SERS signals of the hybrids can be well controlled by adjusting the weight ratio between AgNO(3) and GO. Utilizing GO-AgNPs as the highly sensitive optical probes, fast SERS imaging of cancer cells is realized with a very short integration time of about 0.06 s per pixel. Furthermore, folic acid (FA) is covalently conjugated to GO for targeting specific cancer cells with folate receptors (FRs). Targeted SERS images can be acquired after 2 h incubation with FA-GO-AgNPs, which are specifically located on the surface of FR-positive cancer cells. In conclusion, the GO-based Raman probes mentioned here open up exciting opportunities for biomedical imaging.

摘要

本征 Raman 信号为氧化石墨烯（GO）在细胞成像中的应用提供了潜力。在此，开发了基于 GO-银纳米粒子（NP）复合材料的新型表面增强 Raman 散射（SERS）标记物，用于快速细胞探测和成像。通过调整 AgNO(3)和 GO 的重量比，可以很好地控制混合物的最优 SERS 信号。利用 GO-AgNPs 作为高灵敏度的光学探针，通过约 0.06 s 每像素的非常短的积分时间，实现了癌细胞的快速 SERS 成像。此外，将叶酸（FA）通过共价键连接到 GO 上，用于靶向具有叶酸受体（FRs）的特定癌细胞。在用 FA-GO-AgNPs 孵育 2 h 后，可以获得靶向 SERS 图像，这些探针特异性地位于 FR 阳性癌细胞的表面。总之，这里提到的基于 GO 的 Raman 探针为生物医学成像开辟了令人兴奋的机会。