Department of Dermatology, Osaka City University Graduate School of Medicine, 1-4-3 Asahimachi, Abeno, Osaka 545-8585, Japan ; Brain Science Institute, Osaka City University Graduate School of Medicine, 1-4-3 Asahimachi, Abeno, Osaka 545-8585, Japan.
J Clin Biochem Nutr. 2013 Jan;52(1):58-63. doi: 10.3164/jcbn.12-51. Epub 2012 Nov 20.
This study investigated the mechanism by which the strength and weakness of exercise stress affects the skin symptoms of atopic dermatitis (AD). Specific pathogen-free (SPF) and conventional NC/Nga mice were used. Conventional mice, but not the SPF, spontaneously develop dermal symptoms similar to that of patients with AD. There were two types of stress, mild (20 m/min for 60 min) or strong exercise (25 m/min for 90 min), using a treadmill four times per day. The symptom of the conventional group were strongly exacerbated by strong exercise but ameliorated by mild exercise. The plasma concentrations of α-melanocyte stimulating hormone (α-MSH) and the expression of melanocortin receptor-1 in skin elevated after strong exercise but decreased after mild exercise. The plasma levels of β-endorphin and the expression of µ-opioid receptor in skin were increased by mild exercise. In addition, the expression of prohormone convertase (PC) 1/3, PC2 and carboxypeptidase E (CPE) in pituitary gland were higher in the conventional group than in the SPF group. The level of PC2 was suppressed by mild exercise in the conventional groups, and elevated further by strong exercise. The level of PC1/3 becomes higher with the increase of the exercise load. On the other hand, the expression of the CPE was further increase by mild exercise but suppressed by strong exercise. These observations suggested that exercise-induced stress significantly affect the symptoms of AD in a pivotal manner depending on the levels of α-MSH and β-endorphin, and the expression of pituitary PC2 and CPE.
本研究旨在探讨运动应激的强弱对特应性皮炎（AD）皮肤症状的影响机制。使用无特定病原体（SPF）和常规 NC/Nga 小鼠。常规小鼠而非 SPF 会自发出现类似于 AD 患者的皮肤症状。有两种类型的应激，轻度（20 m/min 持续 60 分钟）或剧烈运动（25 m/min 持续 90 分钟），每天使用跑步机四次。常规组的症状在剧烈运动下强烈恶化，但在轻度运动下得到改善。强运动后，血浆中α-黑素细胞刺激素（α-MSH）浓度和皮肤中黑素皮质素受体-1的表达增加，而弱运动后则降低。弱运动可增加皮肤中β-内啡肽和μ-阿片受体的表达。此外，垂体前叶中前激素转化酶（PC）1/3、PC2 和羧肽酶 E（CPE）的表达在常规组中高于 SPF 组。常规组中，弱运动可抑制 PC2 的表达，而强运动则进一步升高。PC1/3 的水平随着运动负荷的增加而升高。另一方面，CPE 的表达在弱运动时进一步增加，但在强运动时受到抑制。这些观察结果表明，运动引起的应激会显著影响 AD 症状，其方式取决于 α-MSH 和 β-内啡肽的水平以及垂体 PC2 和 CPE 的表达。
