Shafique Rubaba Hamid, Inam Muhammad, Ismail Muhammad, Chaudhary Farhana Riaz
Department of Zoology, Pir Mehr Ali Shah, University of Arid Agriculture Rawalpindi, Rawalpindi, Pakistan.
Allergy Rhinol (Providence). 2012 Fall;3(2):e74-90. doi: 10.2500/ar.2012.3.0036. Epub 2012 Dec 18.
Group 10 allergens (tropomyosins) have been assumed to be a major cause of cross-reactivity between house-dust mites (HDMs) and other invertebrates. Despite all of the published data regarding the epidemiology, percent IgE binding and level of sensitization in the population, the role of tropomyosin as a cross-reactive allergen in patients with multiple allergy syndrome still remains to be elucidated. Homology between amino acid sequences reported in allergen databases of selected invertebrate tropomyosins was determined with Der f 10 as the reference allergen. The 66.9 and 54.4% identities were found with selected crustacean and insect species, respectively, whereas only 20.4% identity was seen with mollusks. A similar analysis was performed using reported B-cell IgE-binding epitopes from Met e1 (shrimp allergen) and Bla g7 (cockroach allergen) with other invertebrate tropomyosins. The percent identity in linear sequences was higher than 35% in mites, crustaceans, and cockroaches. The polar and hydrophobic regions in these groups were highly conserved. These findings suggest that tropomyosin may be a major cause of covariation of sensitization between HDMs, crustaceans, and some species of insects and mollusks.
第10组变应原（原肌球蛋白）被认为是屋尘螨（HDM）与其他无脊椎动物之间交叉反应的主要原因。尽管已发表了所有关于流行病学、人群中IgE结合百分比和致敏水平的数据，但原肌球蛋白作为多重过敏综合征患者交叉反应性变应原的作用仍有待阐明。以Der f 10作为参考变应原，确定了所选无脊椎动物原肌球蛋白变应原数据库中报告的氨基酸序列之间的同源性。分别在所选甲壳类和昆虫物种中发现了66.9%和54.4%的同源性，而在软体动物中仅发现20.4%的同源性。使用已报道的来自Met e1（虾变应原）和Bla g7（蟑螂变应原）的B细胞IgE结合表位与其他无脊椎动物原肌球蛋白进行了类似分析。螨类、甲壳类和蟑螂的线性序列同源百分比高于35%。这些组中的极性和疏水区高度保守。这些发现表明，原肌球蛋白可能是HDM、甲壳类以及某些昆虫和软体动物物种之间致敏共同变化的主要原因。
