Nutrition and Metabolic Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
J Med Food. 2013 Feb;16(2):147-54. doi: 10.1089/jmf.2012.2509. Epub 2013 Jan 23.
In this study, we investigated the effects of genistein, daidzein, and soy protein on paraoxonase and arylesterase activity, malondialdehyde (MDA) levels, and lipid profiles of arthritic rats in vivo and the results were compared with that of dexamethasone. Seventy-two female Sprague-Dawley rats were divided into six groups: healthy control, animals with collagen-induced arthritis (CIA), CIA-soy protein (7 g/kg)-treated rats, CIA-genistein (20 mg/kg)-treated animals, CIA-daidzein (20 mg/kg)-treated rats, and CIA-dexamethasone (1 mg/kg)-treated rats. Rheumatoid arthritis was induced using collagen type II and the treatments were carried out by daily gavages feedings for 50 days. The paraoxonase activity in serum was measured spectrophotometrically using paraoxon and phenylacetate as substrates. Serum MDA and lipids levels were determined using enzymatic colorimetric methods. Arthritis-induced decreases in paraoxonase and arylesterase activity was restored after treatment with soy protein and isoflavones (P<.05). MDA concentrations were lower after treatment with all tested compounds. However, only soy protein could partially improve the lipid profile.
在这项研究中，我们研究了染料木黄酮、大豆苷元和大豆蛋白对体内关节炎大鼠的对氧磷酶和芳基酯酶活性、丙二醛（MDA）水平和脂质谱的影响，并将结果与地塞米松进行了比较。72 只雌性 Sprague-Dawley 大鼠分为六组：健康对照组、胶原诱导关节炎（CIA）动物组、CIA-大豆蛋白（7g/kg）治疗组、CIA-染料木黄酮（20mg/kg）治疗组、CIA-大豆苷元（20mg/kg）治疗组和 CIA-地塞米松（1mg/kg）治疗组。采用 II 型胶原诱导类风湿关节炎，通过每日灌胃给药 50 天进行治疗。采用对氧磷和苯乙酸作为底物，通过分光光度法测量血清中对氧磷酶活性。采用酶比色法测定血清 MDA 和脂质水平。用大豆蛋白和异黄酮治疗后，关节炎诱导的对氧磷酶和芳基酯酶活性降低得到恢复（P<.05）。用所有测试化合物治疗后 MDA 浓度降低，但只有大豆蛋白能部分改善脂质谱。
