Nutrition Research Center, Ahvaz Jondishapour University of Medical Sciences, Ahvaz, Iran.
Bioimpacts. 2011;1(3):161-70. doi: 10.5681/bi.2011.022. Epub 2011 Sep 30.
We have already shown the protective effects of soy protein on rheumatoid arthritis in rats. In this study, the effects of genistein and daidzein, two isoflavones from soy on rheumatoid arthritis prognosis and prevention in rats have been investigated.
Rheumatoid arthritis was induced in female Sprague-Dawley rats using collagen type II plus adjuvant. Rats were then treated with soy protein (7 g/kg), dexamethasone (1 mg/kg), genistein (20 mg/kg genistein), daidzein (20 mg/kg genistein) and casein (in control groups) by daily gavage feedings for 50 days. Scores of arthritis were recorded every day for each paw of animal. Serum concentrations of TNF-α, IL-6, adiponectin and leptin were characterized. Tibiotarsal tissue was used for histopathologic analyses.
Treatment with genistein and daidzein resulted in not only a reduction in disease symptoms but also a delay in the onset of symptoms. Results from delayed-type hypersensitivity test demonstrated that the ear thickness in treated rats was significantly lower than that in the control group (p<0.05). There was a reduction in TNF-α, IL-6, adiponectin and leptin serum concentrations after treatment with genistein and daidzein. Dexamethasone reduced the serum concentrations of TNF-α, IL-6 and adiponectin but increased leptin serum level. Prevention of the tissue damage and joint inflammation was also observed following treatment with two soy isoflavones.
soy isoflavones, daidzein and especially genistein, could significantly improve rheumatoid arthritis symptoms in rats. The structural similarity of isoflavones to estrogen could be the possible underlying mechanism involved in the function.
我们已经证明了大豆蛋白对大鼠类风湿关节炎的保护作用。在这项研究中，研究了大豆中的两种异黄酮染料木黄酮和大豆苷元对大鼠类风湿关节炎预后和预防的影响。
使用 II 型胶原蛋白加佐剂诱导雌性 Sprague-Dawley 大鼠发生类风湿关节炎。然后，通过每日灌胃喂养，用大豆蛋白（7g/kg）、地塞米松（1mg/kg）、染料木黄酮（20mg/kg 染料木黄酮）、大豆苷元（20mg/kg 大豆苷元）和酪蛋白（在对照组中）对大鼠进行治疗，共 50 天。每天记录每只动物每只爪子的关节炎评分。检测 TNF-α、IL-6、脂联素和瘦素的血清浓度。对胫骨组织进行组织病理学分析。
染料木黄酮和大豆苷元的治疗不仅减轻了疾病症状，而且还延迟了症状的发作。迟发型超敏反应试验的结果表明，治疗组大鼠的耳厚明显低于对照组（p<0.05）。治疗后，TNF-α、IL-6、脂联素和瘦素的血清浓度降低。地塞米松降低了 TNF-α、IL-6 和脂联素的血清水平，但增加了瘦素的血清水平。两种大豆异黄酮的治疗还观察到组织损伤和关节炎症的预防。
大豆异黄酮、大豆苷元和特别是染料木黄酮，可以显著改善大鼠的类风湿关节炎症状。异黄酮与雌激素的结构相似可能是其发挥作用的潜在机制。
