Department of Medicine, Hallym University College of Medical School, Hallym University Sacred Heart Hospital, Anyang, Korea.
Dig Dis Sci. 2013 Jun;58(6):1491-6. doi: 10.1007/s10620-012-2555-z. Epub 2013 Jan 24.
Sodium alginate is currently used in medical products, including drugs and cosmetic materials. It can also be used as a submucosal injection material due to its excellent water retention ability. Alginate with a high water retention ability is called alginate hydrogel (AH). The aim of this study was to investigate the usefulness of AH as a submucosal injection material.
To investigate the optimal viscosity of AH as a submucosal injection material, we observed the changes in submucosal height from the initial submucosal height in the stomachs of six miniature pigs for each injection material tested (0.3 % AH, 0.5 % hyaluronic acid, glycerol). All submucosal heights were compared serially over time (3, 5, 10, 20, and 30 min). Both immediate and 1-week delayed tissue reactions were investigated endoscopically in the same living pigs. Histological analyses were performed after the animals had been sacrificed.
In a preliminary study, we determined that 0.3 % sodium alginate mixed with BaCl2 (400 μl) was the optimal viscosity of AH as an injection material. Our comparison of submucosal height changes over time showed that there was a significant decrease in submucosal height just 3 min following the injection of hyaluronic acid and glycerol, but that following the injection of AH a significant decrease in submucosal height was observed only after 10 min (p < 0.05). The histological analyses revealed that there were mild capillary dilations with congestion and mild fibrotic changes with some lymphocytic infiltration at the AH injection site.
Alginate hydrogel demonstrated long-lasting maintenance of submucosal elevation, safety, and cost-effectiveness in a pig model, which makes it a potential alternative to hyaluronic acid.
海藻酸钠目前被应用于医疗产品，包括药物和化妆品材料。由于其优异的保水能力，它也可以作为黏膜下注射材料。保水能力高的海藻酸盐称为海藻酸钠水凝胶（AH）。本研究旨在探讨 AH 作为黏膜下注射材料的有效性。
为了研究 AH 作为黏膜下注射材料的最佳黏度，我们观察了 6 头小型猪每一种注射材料（0.3%AH、0.5%透明质酸、甘油）的黏膜下高度从初始黏膜下高度的变化。所有黏膜下高度均随时间（3、5、10、20 和 30 分钟）进行连续比较。在同一头活猪中，我们还分别进行了即刻和 1 周延迟的组织反应的内镜检查。动物死后进行组织学分析。
在初步研究中，我们确定 0.3%海藻酸钠与 BaCl2（400μl）混合是 AH 作为注射材料的最佳黏度。我们对黏膜下高度随时间的变化进行了比较，结果表明，透明质酸和甘油注射后仅 3 分钟，黏膜下高度就显著下降，而 AH 注射后仅 10 分钟才观察到黏膜下高度显著下降（p<0.05）。组织学分析显示，AH 注射部位有轻微的毛细血管扩张伴充血，轻微的纤维化改变，伴有一些淋巴细胞浸润。
海藻酸钠水凝胶在猪模型中显示出黏膜下隆起的持久维持、安全性和成本效益，使其成为透明质酸的潜在替代品。
