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在小鼠模型中，采用基于修饰树枝状大分子的递送系统配制的H5-DNA疫苗经皮接种后，诱导针对禽流感病毒的强大免疫反应。

Induction of a robust immune response against avian influenza virus following transdermal inoculation with H5-DNA vaccine formulated in modified dendrimer-based delivery system in mouse model.

作者信息

Bahadoran Azadeh, Ebrahimi Mehdi, Yeap Swee Keong, Safi Nikoo, Moeini Hassan, Hair-Bejo Mohd, Hussein Mohd Zobir, Omar Abdul Rahman

机构信息

Institute of Bioscience, Universiti Putra Malaysia, UPM, Serdang.

Department of Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur.

出版信息

Int J Nanomedicine. 2017 Nov 30;12:8573-8585. doi: 10.2147/IJN.S139126. eCollection 2017.

DOI:10.2147/IJN.S139126
PMID:29270010
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5729183/
Abstract

This study was aimed to evaluate the immunogenicity of recombinant plasmid deoxyribonucleic acid (DNA), pBud-H5-green fluorescent protein (GFP)-interferon-regulatory factor (IRF)3 following delivery using polyamidoamine (PAMAM) dendrimer and transactivator of transcription (TAT)-conjugated PAMAM dendrimer as well as the effect of IRF3 as the genetic adjuvant. BALB/c mice were vaccinated transdermally with pBud-H5-GFP, PAMAM/pBud-H5-GFP, TAT-PAMAM/pBud-H5-GFP, and TAT-PAMAM/pBud-H5-GFP-IRF3. The expression analysis of H5 gene from the blood by using quantitative real-time reverse transcriptase polymerase chain reaction confirmed the ability of PAMAM dendrimer as a carrier for gene delivery, as well as the ability of TAT peptide to enhance the delivery efficiency of PAMAM dendrimer. Mice immunized with modified PAMAM by TAT peptide showed higher hemagglutination inhibition titer, and larger CD3/CD4 T cells and CD3/CD8 T cells population, as well as the production of cytokines, namely, interferon (IFN)-γ, interleukin (IL)-2, IL-15, IL-12, IL-6, and tumor necrosis factor-α compared with those immunized with native PAMAM. These results suggest that the function of TAT peptide as a cell-penetrating peptide is able to enhance the gene delivery, which results in rapid distribution of H5 in the tissues of the immunized mice. Furthermore, pBud-H5-GFP co-expressing IRF3 as a genetic adjuvant demonstrated the highest hemagglutination inhibition titer besides larger CD3/CD4 and CD3/CD8 T cells population, and strong Th1-like cytokine responses among all the systems tested. In conclusion, TAT-PAMAM dendrimer-based delivery system with IRF3 as a genetic adjuvant is an attractive transdermal DNA vaccine delivery system utilized to evaluate the efficacy of the developed DNA vaccine in inducing protection during challenge with virulent H5N1 virus.

摘要

本研究旨在评估使用聚酰胺胺（PAMAM）树枝状大分子和转录激活因子（TAT）偶联的PAMAM树枝状大分子递送重组质粒脱氧核糖核酸（DNA）pBud-H5-绿色荧光蛋白（GFP）-干扰素调节因子（IRF）3后的免疫原性，以及IRF3作为遗传佐剂的作用。将BALB/c小鼠经皮接种pBud-H5-GFP、PAMAM/pBud-H5-GFP、TAT-PAMAM/pBud-H5-GFP和TAT-PAMAM/pBud-H5-GFP-IRF3。通过定量实时逆转录聚合酶链反应对血液中H5基因的表达分析证实了PAMAM树枝状大分子作为基因递送载体的能力，以及TAT肽增强PAMAM树枝状大分子递送效率的能力。与用天然PAMAM免疫的小鼠相比，用TAT肽修饰的PAMAM免疫的小鼠表现出更高的血凝抑制效价、更大的CD3/CD4 T细胞和CD3/CD8 T细胞群体，以及细胞因子即干扰素（IFN）-γ、白细胞介素（IL）-2、IL-15、IL-12、IL-6和肿瘤坏死因子-α的产生。这些结果表明，TAT肽作为细胞穿透肽的功能能够增强基因递送，从而导致H5在免疫小鼠组织中的快速分布。此外，共表达IRF3作为遗传佐剂的pBud-H5-GFP在所有测试系统中除了具有更大的CD3/CD4和CD3/CD8 T细胞群体外，还表现出最高的血凝抑制效价和强烈的Th1样细胞因子反应。总之，以IRF3作为遗传佐剂的基于TAT-PAMAM树枝状大分子的递送系统是一种有吸引力的经皮DNA疫苗递送系统，用于评估所开发的DNA疫苗在强毒H5N1病毒攻击期间诱导保护的功效。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fe4/5729183/2baa56772b1e/ijn-12-8573Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fe4/5729183/9299e7e4e79d/ijn-12-8573Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fe4/5729183/21d8ed255590/ijn-12-8573Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fe4/5729183/4e1092d2613b/ijn-12-8573Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fe4/5729183/2e29179ad51e/ijn-12-8573Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fe4/5729183/7b2898a4929a/ijn-12-8573Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fe4/5729183/2baa56772b1e/ijn-12-8573Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fe4/5729183/9299e7e4e79d/ijn-12-8573Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fe4/5729183/21d8ed255590/ijn-12-8573Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fe4/5729183/4e1092d2613b/ijn-12-8573Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fe4/5729183/2e29179ad51e/ijn-12-8573Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fe4/5729183/7b2898a4929a/ijn-12-8573Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fe4/5729183/2baa56772b1e/ijn-12-8573Fig6.jpg

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Pharmaceuticals (Basel). 2022 Jul 16;15(7):877. doi: 10.3390/ph15070877.
5
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