Florencio-Silva R, Santos M A, de Medeiros V P, Nader H B, Nonaka K O, Simões M J, Reginato R D
Department of Morphology and Genetics, Division of Histology and Structural Biology, Federal University of São Paulo , São Paulo.
Climacteric. 2013 Dec;16(6):709-17. doi: 10.3109/13697137.2013.769096. Epub 2013 Feb 27.
To investigate the effects of soy isoflavones (Iso) and mechanical vibration treatments alone or combined on bone extracellular matrix constituents of ovariectomized rats.
Forty female Wistar rats at the age of 6 months were ovariectomized (Ovx) and ten were sham-operated (sham). After 3 months, the animals were divided into five groups: GI (sham); GII (Ovx); GIII, ovariectomized and orally treated with isoflavones (200 mg/kg) for 90 consecutive days; GIV, ovariectomized and submitted to vibration for 90 days (5 days/week); GV, ovariectomized and treated with isoflavones plus vibration. After treatments, the rats were euthanized, and their femurs were removed for histological routine and biochemical study. Histological sections were stained with hematoxylin-eosin, picrosirius red and alcian blue. Shaft of femurs were submitted to biochemical assay and tibias were subjected to biophysical and biomechanical tests.
Treatments did not have significant effects on the trabecular bone volume, but the combined treatments showed trophic effects on the cortical bone width and area. Bone density and the content of organic material of the tibias were higher in the GIV and GV groups. The GV group showed the highest presence of mature collagen fibers and content of total glycosaminoglycans, while the highest contents of chondroitin sulfate and other sulfated glycosaminoglycans were seen in the GIV group.
The mechanical vibration treatment is more efficient than soy isoflavones in improving bone quality by increasing the bone density, the content of sulfated glycosaminoglycans and the presence of mature collagen fibers. In addition, the combined interventions have partial trophic and synergistic effects that are bone site-specific in ovariectomized rats.
研究大豆异黄酮（Iso）及机械振动单独或联合处理对去卵巢大鼠骨细胞外基质成分的影响。
将40只6月龄雌性Wistar大鼠去卵巢（Ovx），10只假手术（sham）。3个月后，动物分为五组：GI（假手术组）；GII（去卵巢组）；GIII，去卵巢并连续90天口服异黄酮（200mg/kg）；GIV，去卵巢并接受90天振动处理（每周5天）；GV，去卵巢并接受异黄酮加振动处理。处理后，处死大鼠，取出股骨进行组织学常规和生化研究。组织学切片用苏木精-伊红、天狼星红和阿尔辛蓝染色。股骨骨干进行生化分析，胫骨进行生物物理和生物力学测试。
处理对小梁骨体积无显著影响，但联合处理对皮质骨宽度和面积有营养作用。GIV组和GV组胫骨的骨密度和有机物质含量较高。GV组成熟胶原纤维的存在和总糖胺聚糖含量最高，而GIV组硫酸软骨素和其他硫酸化糖胺聚糖含量最高。
机械振动处理在提高骨密度、硫酸化糖胺聚糖含量和成熟胶原纤维存在方面比大豆异黄酮更有效地改善骨质量。此外，联合干预在去卵巢大鼠中有部分营养和协同作用，且具有骨部位特异性。
