F Carbonel A A, Azevedo Lima P D, Lim J J, Teixeira Borges F, Rodrigues da Silva Sasso G, Portugal Fuchs L F, S Simões R, Chada Baracat E, Soares J M, J Simões M
a Department of Morphology and Genetics , Paulista School of Medicine/Federal University of São Paulo, EPM/UNIFESP , São Paulo , Brazil.
b Department of Cellular and Molecular Medicine , University of Ottawa , Ottawa , Canada.
Climacteric. 2017 Dec;20(6):564-570. doi: 10.1080/13697137.2017.1366977. Epub 2017 Sep 2.
To assess the effects of isoflavones and 17β-estradiol on the vaginal epithelium extracellular matrix and hyaluronic acid (HA) in the diabetic rat model.
Sixty adult, virgin, female rats underwent ovariectomy, then randomization into six groups of ten animals each: GI, sham ovariectomized control animals; GII, sham ovariectomized control diabetic animals; GIII, control ovariectomized rats receiving propylene glycol vehicle; GIV, control ovariectomized diabetic animals receiving propylene glycol vehicle; GV, diabetic ovariectomized animals treated with soy isoflavones (150 mg/kg by gavage); GVI, ovariectomized diabetic rats treated with estrogen (17β-estradiol, 10 mg/kg, subcutaneously). Treatment took place over 30 consecutive days. After euthanasia, a portion of the vagina was immersed in liquid nitrogen for RT-qPCR and Western blotting. Another portion was processed for paraffin embedding. Sections were stained with hematoxylin & eosin for histomorphometry and Picro Sirius Red for collagen quantification.
Vaginal epithelium histomorphometry in GIII (15.3 ± 1.1 µm) and GIV (14.5 ± 1.8 µm) was thinner than in GV (41.3 ± 1.5 µm) and GVI (74.3 ± 1.6 µm). There was an increase in collagen content in GV (84.1 ± 1.2 µm) and GVI (88.2 ± 1.7 µm). HA quantification was higher in GV (0.38 ± 1.1 μg/mg) and GVI (0.49 ± 1.4 μg/mg) when compared with GIII (0.12 ± 1.1 μg/mg) and GIV (0.10 ± 1.2 μg/mg), p < 0.05.
Soy isoflavones increase hyaluronic acid concentration in the vagina of diabetic ovariectomized rats. Such findings might help to attenuate the effects of vulvovaginal atrophy in women.
评估异黄酮和17β - 雌二醇对糖尿病大鼠模型阴道上皮细胞外基质和透明质酸（HA）的影响。
60只成年未孕雌性大鼠接受卵巢切除术，然后随机分为6组，每组10只动物：GI，假卵巢切除对照动物；GII，假卵巢切除对照糖尿病动物；GIII，接受丙二醇载体的卵巢切除对照大鼠；GIV，接受丙二醇载体的卵巢切除对照糖尿病动物；GV，用大豆异黄酮（150mg/kg灌胃）治疗的糖尿病卵巢切除动物；GVI，用雌激素（17β - 雌二醇，10mg/kg皮下注射）治疗的卵巢切除糖尿病大鼠。连续治疗30天。安乐死后，将一部分阴道浸入液氮中用于RT - qPCR和蛋白质印迹分析。另一部分进行石蜡包埋。切片用苏木精和伊红染色用于组织形态计量学分析，用苦味酸天狼星红染色用于胶原定量分析。
GIII组（15.3±1.1μm）和GIV组（14.5±1.8μm）的阴道上皮组织形态计量学分析显示其厚度比GV组（41.3±1.5μm）和GVI组（74.3±1.6μm）薄。GV组（84.1±1.2μm）和GVI组（88.2±1.7μm）的胶原含量增加。与GIII组（0.12±1.1μg/mg）和GIV组（0.10±1.2μg/mg）相比，GV组（0.38±1.1μg/mg）和GVI组（0.49±1.4μg/mg）的HA定量更高，p<0.05。
大豆异黄酮可增加糖尿病卵巢切除大鼠阴道中的透明质酸浓度。这些发现可能有助于减轻女性外阴阴道萎缩的影响。
