Operative Dentistry Department, Faculty of Dentistry, King AbdulAziz University, Jeddah, Saudi Arabia.
Dent Mater. 2013 Apr;29(4):357-64. doi: 10.1016/j.dental.2012.12.002. Epub 2013 Jan 22.
Phosphoric acid-Bioglass 45S5 paste can create an interaction layer formed of calcium-phosphate crystals on the dentin surface. In this study, the efficiency of decreasing the dentin permeability exerted by the interaction layer formed between bioglass and dentin was compared to a resin-containing oxalate desensitizing agent (MS Coat One) and a resin-free oxalate desensitizing agent (Super Seal).
Dentin permeability was measured before/after a brushing abrasion challenge, followed by examining the top and the fractured dentin surfaces with a field emission scanning electron microscope. Moreover, the chemical nature of the compounds formed on top of the dentin surface was examined using the field emission scanning electron microscope (FE-SEM) equipped with an energy-dispersive X-ray spectroscope (EDS), and the crystalline structures of the dentinal surfaces were examined by X-ray diffraction (XRD).
The results showed that application of 45S5 bioglass paste to dentin was able to occlude patent dentinal tubule orifices with a layer of calcium-phosphate crystals, while the oxalate containing agents were able to form small crystals which were found in dentinal tubule orifices and scattered along the superficial parts of the dentinal tubule lumen. The brushing-abrasion challenge significantly increased the permeability of dentin treated by Super Seal and MS Coat One, while these challenges had no significant effect on the dentin permeability of specimens treated with 45S5 bioglass paste.
The new technique provided better durability than two products available on the market. Moreover, our previous research showed the biocompatibility of using this technique on dental pulp cells, suggesting that this technique can aid in treating dentin hypersensitivity cases.
磷酸-生物玻璃 45S5 糊剂可在牙本质表面形成钙磷晶体的相互作用层。本研究比较了生物玻璃与牙本质形成的相互作用层降低牙本质渗透性的效率与含草酸酯的脱敏剂(MS Coat One)和无树脂的草酸酯脱敏剂(Super Seal)。
在刷牙磨损挑战前后测量牙本质渗透性,然后用场发射扫描电子显微镜检查牙本质表面的顶部和断裂表面。此外,用场发射扫描电子显微镜(FE-SEM)配备能量色散 X 射线光谱仪(EDS)检查牙本质表面形成的化合物的化学性质,并通过 X 射线衍射(XRD)检查牙本质表面的晶体结构。
结果表明,将 45S5 生物玻璃糊剂应用于牙本质能够用一层钙磷晶体封闭有孔的牙本质小管口,而含草酸酯的试剂能够形成小晶体,这些小晶体位于牙本质小管口内,并沿着牙本质小管腔的浅表部分散布。刷牙磨损挑战显著增加了 Super Seal 和 MS Coat One 处理的牙本质的渗透性,而这些挑战对用 45S5 生物玻璃糊剂处理的牙本质的渗透性没有显著影响。
新技术提供的耐久性优于市场上两种现有产品。此外,我们之前的研究表明,在牙髓细胞上使用这种技术具有生物相容性,表明该技术可以帮助治疗牙本质过敏病例。