Department of Biomedical Engineering, University of Alabama at Birmingham, USA.
IEEE Trans Ultrason Ferroelectr Freq Control. 2013 Feb;60(2):281-9. doi: 10.1109/TUFFC.2013.2564.
Microbubble-mediated ultrasound therapy can noninvasively enhance drug delivery to localized regions in the body. This technique can be beneficial in cancer therapy, but currently there are limitations to tracking the therapeutic effects. The purpose of this experiment was to investigate the potential of fluorescent imaging for monitoring the temporal effects of microbubble-mediated ultrasound therapy. Mice were implanted with 2LMP breast cancer cells. The animals underwent microbubble-mediated ultrasound therapy in the presence of Cy5.5 fluorescent-labeled IgG antibody (large molecule) or Cy5.5 dye (small molecule) and microbubble contrast agents. Control animals were administered fluorescent molecules only. Animals were transiently imaged in vivo at 1, 10, 30, and 60 min post therapy using a small animal optical imaging system. Tumors were excised and analyzed ex vivo. Tumors were homogenized and emulsion imaged for Cy5.5 fluorescence. Monitoring in vivo results showed significant influx of dye into the tumor (p < 0.05) using the small molecule, but not in the large molecule group (p > 0.05). However, after tumor emulsion, significantly higher dye concentration was detected in therapy group tumors for both small and large molecule groups in comparison to their control counterparts (p <0.01). This paper explores a noninvasive optical imaging method for monitoring the effects of microbubble-mediated ultrasound therapy in a cancer model. It provides temporal information following the process of increasing extravasation of molecules into target tumors.
微泡介导的超声治疗可以无创地增强药物向体内局部区域的递送。这种技术在癌症治疗中可能很有帮助，但目前在跟踪治疗效果方面存在限制。本实验旨在探讨荧光成像监测微泡介导的超声治疗时间效应的潜力。将 2LMP 乳腺癌细胞植入小鼠。在存在 Cy5.5 荧光标记 IgG 抗体（大分子）或 Cy5.5 染料（小分子）和微泡造影剂的情况下，对动物进行微泡介导的超声治疗。对照动物仅给予荧光分子。治疗后 1、10、30 和 60 分钟，使用小动物光学成像系统对动物进行短暂的体内成像。切除肿瘤并进行离体分析。将肿瘤匀浆并进行乳液成像以检测 Cy5.5 荧光。体内监测结果显示，小分子组肿瘤内染料显著内流（p<0.05），但大分子组则无（p>0.05）。然而，在肿瘤乳液后，与对照组相比，治疗组中小分子和大分子组的肿瘤中染料浓度均显著升高（p<0.01）。本文探讨了一种非侵入性光学成像方法，用于监测癌症模型中微泡介导的超声治疗效果。它提供了分子进入靶肿瘤的外渗过程的时间信息。
