Department of Biosciences, COMSATS Institute of Information Technology, Park Road, Islamabad, Pakistan.
Am J Trop Med Hyg. 2013 Mar;88(3):519-25. doi: 10.4269/ajtmh.12-0365. Epub 2013 Jan 28.
This study compares Escherichia coli pathotypes circulating among children in Pakistan during the floods of 2010 and 2011 and from sporadic cases outside flood affected areas. Using multiplex polymerase chain reaction 115 of 205 stool samples (56.29%) were positive for diarrheagenic E. coli from specimens taken during the floods compared with 50 of 400 (12.5%) stool samples being positive for sporadic cases. The E. coli pathotypes were categorized as Enteropathogenic E. coli 33 (28.69%) and 13 (26%), Enterotoxigenic E. coli 29 (25.21%) and 15 (30%), Enteroaggregative E. coli 21 (18.2%) and 18 (36%), Enterohemorrhagic E. coli 5 (4.34%) and 1 (2%) from flood and sporadic cases, respectively. Furthermore, patients co-infected with more than one pathotype were 26 (22.60%) and 3 (6%) from flood and sporadic cases, respectively. The study shows an unexpectedly high rate of isolation of E. coli pathotypes suggesting Pakistan as an endemic region that requires active surveillance particularly during flood periods.
本研究比较了 2010 年和 2011 年巴基斯坦洪水中以及洪灾影响地区以外散发性病例中流行的大肠杆菌血清型。使用多重聚合酶链反应，从洪水期间采集的 205 份粪便样本中，有 115 份（56.29%）为腹泻性大肠杆菌呈阳性，而从 400 份散发性病例粪便样本中，有 50 份（12.5%）为阳性。将大肠杆菌血清型分为肠致病性大肠杆菌 33 株（28.69%）和 13 株（26%）、肠毒性大肠杆菌 29 株（25.21%）和 15 株（30%）、肠聚集性大肠杆菌 21 株（18.2%）和 18 株（36%）、肠出血性大肠杆菌 5 株（4.34%）和 1 株（2%），分别来自洪水和散发性病例。此外，有 26 名（22.60%）和 3 名（6%）患者同时感染了一种以上的血清型，分别来自洪水和散发性病例。研究表明，大肠杆菌血清型的分离率出乎意料地高，表明巴基斯坦是一个流行地区，特别是在洪水期间需要进行积极的监测。
