Aranda Katia R S, Fabbricotti Sandra H, Fagundes-Neto Ulysses, Scaletsky Isabel C A
Departamento de Microbiologia, Imunologia e Parasitologia, São Paulo, SP, Brazil.
FEMS Microbiol Lett. 2007 Feb;267(2):145-50. doi: 10.1111/j.1574-6968.2006.00580.x.
A multiplex PCR to differentiate typical and atypical enteropathogenic Escherichia coli (EPEC), enteroaggregative E. coli (EAEC), enterotoxigenic (ETEC), enteroinvasive E. coli (EIEC) and Shiga toxin-producing E. coli (STEC) strains was developed and evaluated. The targets selected for each group were eae and bfpA for EPEC, aggR for EAEC, elt and est for ETEC, ipaH for EIEC and stx for STEC isolates. This PCR was specific and sensitive for rapid detection of target isolates in stools. Among 79 children with acute diarrhea, this technique identified 13 (16.4%) with atypical EPEC, four (5%) with EAEC, three (3.8%) with typical EPEC, one (1.3%) with ETEC and one (1.3%) with EIEC.
开发并评估了一种多重聚合酶链反应（PCR），用于区分典型和非典型肠致病性大肠杆菌（EPEC）、肠聚集性大肠杆菌（EAEC）、产肠毒素大肠杆菌（ETEC）、肠侵袭性大肠杆菌（EIEC）和产志贺毒素大肠杆菌（STEC）菌株。为每组选择的靶标分别是EPEC的eae和bfpA、EAEC的aggR、ETEC的elt和est、EIEC的ipaH以及STEC分离株的stx。该PCR对粪便中靶标分离株的快速检测具有特异性和敏感性。在79名急性腹泻儿童中，该技术鉴定出13例（16.4%）非典型EPEC、4例（5%）EAEC、3例（3.8%）典型EPEC、1例（1.3%）ETEC和1例（1.3%）EIEC。
