Monici Monica, Cialdai Francesca, Ranaldi Francesco, Paoli Paolo, Boscaro Francesca, Moneti Gloriano, Caselli Anna
ASAcampus Joint Laboratory, ASA Res. Div., Dept. Clinical Physiopathology, University of Florence, Italy.
Mol Biosyst. 2013 Jun;9(6):1147-61. doi: 10.1039/c2mb25398d.
Laser therapy is used in physical medicine and rehabilitation to accelerate muscle recovery and in sports medicine to prevent damages produced by metabolic disturbances and inflammatory reactions after heavy exercise. The aim of this research was to get insight into possible benefits deriving from the application of an advanced IR laser system to counteract deficits of muscle energy metabolism and stimulate the recovery of hypotrophic tissue. We studied the effect of IR laser treatment on proliferation, differentiation, cytoskeleton organization and global protein expression in C2C12 myoblasts. We found that laser treatment induced a decrease in the cell proliferation rate without affecting cell viability, while leading to cytoskeletal rearrangement and expression of the early differentiation marker MyoD. The differential proteome analysis revealed the up-regulation and/or modulation of many proteins known to be involved in cell cycle regulation, cytoskeleton organization and differentiation.
激光疗法在物理医学与康复领域用于加速肌肉恢复，在运动医学中用于预防高强度运动后代谢紊乱和炎症反应所产生的损伤。本研究的目的是深入了解应用先进的红外激光系统可能带来的益处，以抵消肌肉能量代谢不足并刺激萎缩组织的恢复。我们研究了红外激光治疗对C2C12成肌细胞增殖、分化、细胞骨架组织和整体蛋白质表达的影响。我们发现激光治疗可降低细胞增殖速率而不影响细胞活力，同时导致细胞骨架重排并使早期分化标志物MyoD表达。差异蛋白质组分析揭示了许多已知参与细胞周期调控、细胞骨架组织和分化的蛋白质的上调和/或调节。
