Razzaghi Mohammadreza, Rostami-Nejad Mohammad, Rezaei-Taviran Mostafa, Zamanian Azodi Mona, Okhovatian Farshad, Mansouri Vahid, Ahmadi Nayebali
Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Lasers Med Sci. 2019 Fall;10(Suppl 1):S49-S53. doi: 10.15171/jlms.2019.S9. Epub 2019 Dec 1.
In sports medicine, laser application has been well-established for the recovery of muscles. The mechanisms by which benefits of this kind of therapy can be studied is molecular research approach. Protein-protein interaction network analysis as one of the important complementary studies of proteomics can accelerate this goal by the identification of novel contributing markers. By the use of Cytoscape V3.7.1 and its applications, a network of differential expressed proteins (DEPs) from IR laser treatment samples were constructed and analyzed. Six hub-bottlenecks were determined, 4 of which were from differentially expressed proteins. ClueGO discovered 4 biological processes related to these hub-bottlenecks that their function could alter due to IR laser therapy. In fact, by the expression changes of hub-bottlenecks including the up-regulation of HSP90s, one of the prominent biological processes in muscle recovery could be activated. This process is called nitric oxide synthase (NOS) activation that could be proposed as one of the underlying mechanisms of IR laser treatments in muscle recovery.
在运动医学中，激光应用在肌肉恢复方面已得到充分确立。研究这种疗法益处的机制是分子研究方法。蛋白质 - 蛋白质相互作用网络分析作为蛋白质组学的重要补充研究之一，可以通过鉴定新的贡献标志物来加速这一目标。通过使用Cytoscape V3.7.1及其应用程序，构建并分析了来自红外激光治疗样本的差异表达蛋白（DEP）网络。确定了六个枢纽瓶颈，其中四个来自差异表达蛋白。ClueGO发现了与这些枢纽瓶颈相关的四个生物学过程，其功能可能因红外激光疗法而改变。事实上，通过枢纽瓶颈的表达变化，包括HSP90s的上调，可以激活肌肉恢复中一个突出的生物学过程。这个过程称为一氧化氮合酶（NOS）激活，这可以被认为是红外激光治疗肌肉恢复的潜在机制之一。
