Santaniello Sabato, Gale John T, Montgomery Erwin B, Sarma Sridevi V
Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
Annu Int Conf IEEE Eng Med Biol Soc. 2012;2012:1214-7. doi: 10.1109/EMBC.2012.6346155.
Despite a pivotal role in the motor loop, dorsolateral striatum (putamen) has been poorly studied thus far under Parkinsonian conditions and Deep Brain Stimulation (DBS). We analyze the activity of the putamen in a monkey by combining single unit recordings and point process models. The animal received DBS (30-130 Hz) in the subthalamic nucleus (STN) while at rest and recordings were acquired both before and after treatment with 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP), which induced Parkinsonian-like motor disorders. 141 neurons were collected and, for each neuron, a point process model captured DBS-evoked discharge patterns. In the normal animal, spike trains at rest had Poisson like distribution with non-stationary recurrent patterns (RPs) of period 3-7 ms and were mildly changed by low frequency (LF, i.e., < 100 Hz) DBS (i.e., < 20% of neurons affected). With high frequency (HF, i.e., 100-130 Hz) DBS, instead, up to 59% of neurons were affected, the DBS history significantly impacted the neuronal spiking propensity, and the RPs and the post-stimulus activation latency decreased. MPTP evoked inter-neuronal dependencies (INDs) at rest and, compared to normal, LF DBS of the MPTP animal increased RPs and INDs, while HF DBS elicited a faster and wider post-stimulus activation. Overall, HF DBS reduced ongoing non-stationary dynamics by regularizing the discharge patterns both in MPTP and normal putamen, while the combination of MPTP and LF DBS enhanced such dynamics.
尽管背外侧纹状体（壳核）在运动环路中起关键作用，但迄今为止，在帕金森病状态和深部脑刺激（DBS）下对其研究甚少。我们通过结合单神经元记录和点过程模型来分析猴子壳核的活动。动物在静息状态下接受丘脑底核（STN）的DBS（30 - 130Hz），并在使用1 - 甲基 - 4 - 苯基 - 1,2,3,6 - 四氢吡啶（MPTP）治疗前后进行记录，MPTP可诱发帕金森样运动障碍。收集了141个神经元，并且为每个神经元建立了一个点过程模型来捕捉DBS诱发的放电模式。在正常动物中，静息时的动作电位序列具有泊松分布，具有3 - 7毫秒的非平稳递归模式（RPs），并且低频（LF，即<100Hz）DBS对其影响较小（即<20%的神经元受影响）。相反，高频（HF，即100 - 130Hz）DBS时，高达59%的神经元受影响，DBS的历史记录显著影响神经元的放电倾向，RPs和刺激后激活潜伏期缩短。MPTP在静息时诱发神经元间依赖性（INDs），与正常情况相比，MPTP处理动物的LF DBS增加了RPs和INDs，而HF DBS引发了更快、更广泛的刺激后激活。总体而言，HF DBS通过规范MPTP处理和正常壳核中的放电模式来减少持续的非平稳动态，而MPTP和LF DBS的组合增强了这种动态。
