Service de Parasitologie-Mycologie, Centre Hospitalier Universitaire de Toulouse, Toulouse, France.
Med Mycol. 2013 Aug;51(6):652-6. doi: 10.3109/13693786.2012.762607. Epub 2013 Feb 4.
Candida spp. are an important cause of nosocomial bloodstream infections. Currently, complete identification of yeasts with conventional methods takes several days. We report here the first evaluation of an extraction method associated with the Vitek MS matrix-assisted laser desorption ionization time of flight mass spectrometry for direct identification of Candida species from positive blood cultures. We evaluated this protocol with blood cultures that were inoculated with reference and routine isolates (eight reference strains, 30 patients isolates and six mixed cultures containing two strains of different Candida species), or from patients with candidemia (28 isolates). This method performed extremely well (97% correct identification) with blood cultures of single Candida spp. and significantly reduced the time of diagnosis. Nevertheless, subculture remains indispensable to test fungal resistance and to detect mixed infections.
念珠菌属是医院获得性血流感染的重要原因。目前，传统方法鉴定酵母菌需要数天时间。我们在此报告了一种与 Vitek MS 基质辅助激光解吸电离飞行时间质谱联用的提取方法的首次评估，该方法可直接从阳性血培养物中鉴定出念珠菌属。我们用接种了参考和常规分离株的血培养物（8 株参考株、30 株患者分离株和 6 株含两种不同念珠菌属菌株的混合培养物）或来自念珠菌血症患者的血培养物（28 株）评估了该方案。该方法对单一念珠菌属的血培养物具有极好的（97%的正确鉴定）性能，并显著缩短了诊断时间。然而，为了检测真菌耐药性和混合感染，仍然需要进行亚培养。
