Animal Infectious Disease Laboratory, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
Vet Microbiol. 2013 May 3;163(3-4):351-7. doi: 10.1016/j.vetmic.2012.12.025. Epub 2013 Jan 17.
One H5N8 and three H5N5 highly pathogenic avian influenza (HPAI) viruses which derived their HA genes from the Asian H5N1 lineage were isolated from poultry during 2009-2010 in mainland China. Pathogenicity studies showed that these viruses were all highly virulent to chickens, while they varied from moderate to high virulence in mice and from mild to intermediate virulence in mallards. Phylogenetic analyses showed that these viruses were reassortants bearing the H5N1 backbone while acquiring PB1, NP and NA genes from unidentified non-H5N1 viruses, and had developed into three distinct genotypes (B-D). Molecular characterization indicated that all these viruses might resist to antiviral agents. Our findings highlight the emergence and development of HPAI H5 viruses of other NA subtypes in H5N1 endemic areas and their potential threat to poultry industry and public health.
在中国大陆，2009 年至 2010 年间，从家禽中分离到了 1 株 H5N8 和 3 株 H5N5 高致病性禽流感（HPAI）病毒，它们的 HA 基因均源自亚洲 H5N1 谱系。致病性研究表明，这些病毒对鸡均具有高度致病性，而对小鼠的致病性则从中等至高不等，对野鸭的致病性则从轻度至中度不等。系统进化分析表明，这些病毒是重配病毒，带有 H5N1 骨架，同时从未鉴定的非 H5N1 病毒中获得了 PB1、NP 和 NA 基因，并已发展成三种不同的基因型（B-D）。分子特征表明，所有这些病毒可能对抗病毒药物有耐药性。我们的研究结果强调了在 H5N1 流行地区出现和发展其他 NA 亚型的 HPAI H5 病毒及其对家禽业和公共卫生的潜在威胁。
