Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, Germany.
Int J Med Microbiol. 2013 Mar;303(2):88-9. doi: 10.1016/j.ijmm.2012.12.007. Epub 2013 Feb 1.
Three clinical A. baumannii isolates Ab-508, Ab-511, and Ab-653 were recovered from South Africa, South Korea, and Turkey, respectively. Multiplex PCR to detect OXA-type carbapenemases showed atypical blaOXA-51-like amplification products. The aim of this study was to investigate the background of changes in blaOXA-51-like PCR products. Isolates were confirmed as A. baumannii using gyrB multiplex and rpoB sequencing and were epidemiologically unrelated by rep-PCR-based DiversiLab. Sequencing of blaOXA-51-like revealed an insertion of ISAba15 in blaOXA-66 (isolate Ab-511) and an insertion of the novel ISAba19 in blaOXA-78 (isolates Ab-508 and Ab-653). Detection of the intrinsic blaOXA-51-like by OXA-multiplex PCR should not be considered a fully reliable method for identification of A. baumannii when used without an additional independent method. Other species identification methods such as gyrB multiplex PCR and rpoB sequencing should be used to reliably identify A. baumannii.
从南非、韩国和土耳其分别回收了三个临床 A.baumannii 分离株 Ab-508、Ab-511 和 Ab-653。用于检测 OXA 型碳青霉烯酶的多重 PCR 显示出非典型的 blaOXA-51 样扩增产物。本研究旨在探讨 blaOXA-51 样 PCR 产物变化的背景。使用 gyrB 多重和 rpoB 测序确认分离株为 A.baumannii，并通过基于 rep-PCR 的 DiversiLab 进行流行病学上无关的分析。blaOXA-51 样的测序显示 blaOXA-66 中的 ISAba15 插入（分离株 Ab-511）和 blaOXA-78 中的新型 ISAba19 插入（分离株 Ab-508 和 Ab-653）。当没有额外的独立方法时，OXA-多重 PCR 检测固有 blaOXA-51 样不应被认为是鉴定 A.baumannii 的完全可靠方法。应使用其他物种鉴定方法，如 gyrB 多重 PCR 和 rpoB 测序，以可靠地鉴定 A.baumannii。
