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J Antimicrob Chemother. 2014 Dec;69(12):3437-8. doi: 10.1093/jac/dku306. Epub 2014 Aug 4.
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Interspecies transfer of blaIMP-4 in a patient with prolonged colonization by IMP-4-producing Enterobacteriaceae.产IMP-4肠杆菌科细菌长期定植患者中blaIMP-4的种间转移
J Clin Microbiol. 2014 Oct;52(10):3816-8. doi: 10.1128/JCM.01491-14. Epub 2014 Jul 23.
3
The SEED and the Rapid Annotation of microbial genomes using Subsystems Technology (RAST).SEED 与利用子系统技术进行快速微生物基因组注释（RAST）。
Nucleic Acids Res. 2014 Jan;42(Database issue):D206-14. doi: 10.1093/nar/gkt1226. Epub 2013 Nov 29.
4
Multiplex PCR to detect the genes encoding naturally occurring oxacillinases in Acinetobacter spp.多重 PCR 检测不动杆菌属中编码天然耐甲氧西林青霉素酶的基因
J Antimicrob Chemother. 2014 Apr;69(4):959-63. doi: 10.1093/jac/dkt480. Epub 2013 Nov 27.
5
Acinetobacter pittii and Acinetobacter nosocomialis among clinical isolates of the Acinetobacter calcoaceticus-baumannii complex in Sichuan, China.在中国四川，鲍曼不动杆菌复合群临床分离株中检出的皮氏不动杆菌和鲍氏不动杆菌。
Diagn Microbiol Infect Dis. 2013 Jul;76(3):392-5. doi: 10.1016/j.diagmicrobio.2013.03.020. Epub 2013 Apr 29.
6
OXA-235, a novel class D β-lactamase involved in resistance to carbapenems in Acinetobacter baumannii.鲍曼不动杆菌碳青霉烯类耐药中涉及的一种新型 D 类β-内酰胺酶 OXA-235。
Antimicrob Agents Chemother. 2013 May;57(5):2121-6. doi: 10.1128/AAC.02413-12. Epub 2013 Feb 25.
7
Rapid identification of carbapenemase types in Enterobacteriaceae and Pseudomonas spp. by using a biochemical test.采用生化试验快速鉴定肠杆菌科和铜绿假单胞菌的碳青霉烯酶类型。
Antimicrob Agents Chemother. 2012 Dec;56(12):6437-40. doi: 10.1128/AAC.01395-12. Epub 2012 Oct 15.
8
Dissemination and characterization of NDM-1-producing Acinetobacter pittii in an intensive care unit in China.中国重症监护病房中产 NDM-1 型金属β-内酰胺酶的鲍曼不动杆菌的传播和特征。
Clin Microbiol Infect. 2012 Dec;18(12):E506-13. doi: 10.1111/1469-0691.12035. Epub 2012 Oct 5.
9
Molecular epidemiology of Acinetobacter baumannii and Acinetobacter nosocomialis in Germany over a 5-year period (2005-2009).德国 5 年间（2005-2009 年）鲍曼不动杆菌和医院不动杆菌的分子流行病学研究。
Clin Microbiol Infect. 2013 Aug;19(8):737-42. doi: 10.1111/1469-0691.12026. Epub 2012 Oct 3.
10
Regional dissemination of Acinetobacter species harbouring metallo-β-lactamase genes in Japan.日本携带金属β-内酰胺酶基因的不动杆菌种的区域传播。
Clin Microbiol Infect. 2013 Aug;19(8):729-36. doi: 10.1111/1469-0691.12013. Epub 2012 Sep 25.

澳大利亚一例产IMP-4、OXA-421、OXA-96和CARB-2的皮特不动杆菌序列型119病例。

A case of IMP-4-, OXA-421-, OXA-96-, and CARB-2-producing Acinetobacter pittii sequence type 119 in Australia.

作者信息

Kamolvit Witchuda, Derrington Petra, Paterson David L, Sidjabat Hanna E

机构信息

The University of Queensland, UQ Centre for Clinical Research, Brisbane, Queensland, Australia.

Pathology Queensland, Herston, Queensland, Australia.

出版信息

J Clin Microbiol. 2015 Feb;53(2):727-30. doi: 10.1128/JCM.02726-14. Epub 2014 Nov 26.

DOI:10.1128/JCM.02726-14
PMID:25428154
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4298557/
Abstract

An IMP-4-producing Acinetobacter pittii strain coproducing oxacillinases was isolated from a leg wound of a 67-year-old female patient. Identification to the species level by rpoB and gyrB sequencing and multiplex-PCR-based analysis revealed that the isolate was A. pittii. Whole-genome sequencing of this A. pittii isolate determined the presence of blaOXA-96, blaCARB-2, and a novel blaOXA-421 gene. The position of this novel blaOXA-421 gene was similar to that of blaOXA-51 in A. baumannii, downstream of the phosphinothricin N-acetyltransferase gene and upstream of fxsA in the chromosome. This A. pittii isolate was found to belong to sequence type 119 (ST119). Here, we report the first isolation of IMP-4-producing A. pittii ST119 with a novel blaOXA-421 gene from a patient in Australia and characterize its draft genome.

摘要

从一名67岁女性患者的腿部伤口分离出一株产IMP-4的皮氏不动杆菌，该菌株同时产奥沙西林酶。通过rpoB和gyrB测序以及基于多重PCR的分析在种水平上鉴定表明，该分离株为皮氏不动杆菌。对该皮氏不动杆菌分离株进行全基因组测序确定存在blaOXA-96、blaCARB-2和一个新的blaOXA-421基因。这个新的blaOXA-421基因的位置与鲍曼不动杆菌中blaOXA-51的位置相似，位于染色体上膦丝菌素N-乙酰转移酶基因下游和fxsA上游。发现该皮氏不动杆菌分离株属于序列型119（ST119）。在此，我们报道了在澳大利亚首次从一名患者中分离出产IMP-4的携带新blaOXA-421基因的皮氏不动杆菌ST119，并对其草图基因组进行了表征。