Montané M H, Dupille E, Alibert G, Teissié J
INPT-Ecole Nationale Supérieure Agronomique de Toulouse, France.
Biochim Biophys Acta. 1990 May 9;1024(1):203-7. doi: 10.1016/0005-2736(90)90227-f.
Electropermeabilized tobacco mesophyll protoplasts are shown to fuse by creating cell contact several minutes after electropulsation. Electropermeabilization was analysed by measuring calcein uptake. Experiments were performed at low temperature to avoid resealing of protoplast transient permeation structures. These results confirm that the long-lived permeabilized state induced by the electric field is associated to a fusogenic state, under viability conditions. This is indicative that as for mammalian cells, the electric field-induced membrane modifications, which give the permeable state, are such as to decrease the magnitude of the intercellular repulsive forces between plant protoplasts. Such a fusion method may be useful for somatic hybrids production with protoplasts showing morphological and physiological differences.
电穿孔处理后的烟草叶肉原生质体在电脉冲处理几分钟后通过细胞接触实现融合。通过测量钙黄绿素摄取量来分析电穿孔情况。实验在低温下进行，以避免原生质体瞬时渗透结构的重新封闭。这些结果证实，在存活条件下，电场诱导的长寿命通透状态与融合状态相关。这表明，与哺乳动物细胞一样，电场诱导的导致通透状态的膜修饰能够降低植物原生质体之间细胞间排斥力的大小。这种融合方法可能有助于利用表现出形态和生理差异的原生质体生产体细胞杂种。
