链脲佐菌素诱导的糖尿病小鼠组织中的碳水化合物含量、糖苷酶和糖基转移酶活性

Carbohydrate contents, and glycosidase and glycosyl transferase activities in tissues from streptozotocin diabetic mice.

作者信息

Serrano M A, Cabezas J A, Reglero A

出版信息

Comp Biochem Physiol B. 1985;80(3):629-32. doi: 10.1016/0305-0491(85)90303-7.

Abstract

The contents of hexoses and hexosamines in brain, liver, and kidney of streptozotocin diabetic mice are significantly increased in comparison to the controls. These differences for hexoses contents in the heart are not significant. N-acetyl-beta-D-glucosaminidase and beta-D-glucosidase activities in brain, liver and kidney of diabetic mice are significantly higher when compared to the controls. However, beta-D-galactosidase activity is significantly lower in brain, liver, spleen and kidney of the diabetic mice, in comparison to the controls and similar in heart. alpha-D-Mannosidase activity of diabetic mice is significantly increased in spleen and heart and significantly decreased in liver and kidney. alpha-L-Fucosidase of diabetic mice shows higher activities, with significant differences, in liver and spleen; however, in heart and kidney the activities are significantly lower. Brain sialyltransferase and galactosyltransferase activities are significantly increased in diabetic mice; but for heart and kidney these differences are not significant. The activity for brain and kidney fucosyltransferase is not significant and that for the other assayed organs is significantly higher in comparison to the controls.

摘要

与对照组相比，链脲佐菌素诱导的糖尿病小鼠脑、肝和肾中的己糖和氨基己糖含量显著增加。心脏中己糖含量的这些差异不显著。与对照组相比，糖尿病小鼠脑、肝和肾中的N-乙酰-β-D-氨基葡萄糖苷酶和β-D-葡萄糖苷酶活性显著更高。然而，与对照组相比，糖尿病小鼠脑、肝、脾和肾中的β-D-半乳糖苷酶活性显著降低，心脏中的β-D-半乳糖苷酶活性与对照组相似。糖尿病小鼠的α-D-甘露糖苷酶活性在脾脏和心脏中显著增加，在肝脏和肾脏中显著降低。糖尿病小鼠的α-L-岩藻糖苷酶在肝脏和脾脏中表现出较高的活性，且有显著差异；然而，在心脏和肾脏中，其活性显著降低。糖尿病小鼠脑唾液酸转移酶和半乳糖基转移酶活性显著增加；但心脏和肾脏中的这些差异不显著。脑和肾岩藻糖基转移酶的活性不显著，与对照组相比，其他检测器官中的岩藻糖基转移酶活性显著更高。