Key Laboratory for Space Biosciences & Biotechnology, Institute of Special Environmental Biophysics, School of Life Sciences, Northwestern Polytechnical University Xi'an, Xi'an, China.
PLoS One. 2013;8(1):e51036. doi: 10.1371/journal.pone.0051036. Epub 2013 Jan 29.
The superconducting magnet generates a field and field gradient product that can levitate diamagnetic materials. In this study a specially designed superconducting magnet with a large gradient high magnetic field (LG-HMF), which can provide three apparent gravity levels (μ-g, 1-g, and 2-g), was used to simulate a space-like gravity environment. The effects of LG-HMF on the ultrastructure and function of osteoblast-like cells (MG-63 and MC3T3-E1) and the underlying mechanism were investigated by transmission electromicroscopy (TEM), MTT, and cell western (ICW) assays. Under LG-HMF significant morphologic changes in osteoblast-like cells occurred, including expansion of endoplasmic reticulum and mitochondria, an increased number of lysosomes, distorted microvilli, and aggregates of actin filaments. Compared to controls, cell viability and alkaline phosphatase (ALP) secretion were significantly increased, and collagen I (col I), fibronectin (FN), vinculin, integrin α3, αv, and β1 expression were changed under LG-HMF conditions. In conclusion, LG-HMF affects osteoblast ultrastructure, cell viability, and ALP secretion, and the changes caused by LG-HMF may be related to disrupting col I or FN/αβ1 integrin.
超导磁体产生磁场和磁场梯度乘积，可以使抗磁性材料悬浮。在这项研究中，使用了一种特殊设计的具有大梯度高磁场（LG-HMF）的超导磁体，它可以提供三个明显的重力水平（μ-g、1-g 和 2-g），以模拟太空般的重力环境。通过透射电镜（TEM）、MTT 和细胞西方（ICW）分析研究了 LG-HMF 对成骨样细胞（MG-63 和 MC3T3-E1）的超微结构和功能的影响及其潜在机制。在 LG-HMF 下，成骨样细胞发生了明显的形态变化，包括内质网和线粒体扩张、溶酶体数量增加、微绒毛扭曲和肌动蛋白丝聚集。与对照组相比，细胞活力和碱性磷酸酶（ALP）分泌显著增加，在 LG-HMF 条件下，胶原蛋白 I（col I）、纤维连接蛋白（FN）、 vinculin、整合素α3、αv 和β1 的表达发生变化。总之，LG-HMF 影响成骨细胞的超微结构、细胞活力和 ALP 分泌，LG-HMF 引起的变化可能与破坏 col I 或 FN/αβ1 整合素有关。
