Institute of Cell Biology and Genetics, College of Life Sciences, Zhejiang University, Room 307, Building of Life Sciences, Zijingang Campus, No.388, Yuhangtang Road, Hangzhou, Zhejiang, 310058, People's Republic of China.
Stem Cell Rev Rep. 2010 Dec;6(4):567-78. doi: 10.1007/s12015-010-9182-x.
Microgravity (MG) leads to a decrease in osteogenic potential of human bone marrow-derived mesenchymal stem cells (hMSCs). In the present study, we used large gradient high magnetic field (LGHMF) produced by a superconducting magnet to model MG (LGHMF-MG) and analyzed the effects of LGHMF-MG on survival, cytoskeleton and osteogenic potential of hMSCs. Results showed that the LGHMF-MG treatment for 6 h disrupted the cytoskeleton of hMSCs, and the LGHMF-MG treatment for 24 h led to cell death. LGHMF-MG treatments for 6 h in early stages of osteogenic induction (the pre-treatment before osteogenic induction, the beginning-treatment in the beginning-stage of osteogenic induction and the middle-treatment in the middle-stage of osteogenic induction) resulted in suppression on osteogenesis of hMSCs. The suppression intensity was reduced gradually as the treatment stage of LGHMF-MG was postponed. The LGHMF-MG treatment for 6 h in the ending-stage of osteogenic induction (the ending-treatment) had no obvious effect on osteogenesis of hMSCs. These results indicated that LGHMF-MG should affect the initiation of osteogenesis. Finally, the possible mechanism for the inhibition effect of LGHMF-MG on osteogenesis of hMSCs is discussed.
微重力（MG）会降低人骨髓间充质干细胞（hMSCs）的成骨潜能。在本研究中，我们使用超导磁体产生的大梯度强磁场（LGHMF）来模拟 MG（LGHMF-MG），并分析了 LGHMF-MG 对 hMSCs 存活、细胞骨架和成骨潜能的影响。结果表明，LGHMF-MG 处理 6 小时会破坏 hMSCs 的细胞骨架，而 LGHMF-MG 处理 24 小时会导致细胞死亡。在成骨诱导的早期阶段（成骨诱导前的预处理、成骨诱导早期阶段的开始处理和成骨诱导中期阶段的中间处理），6 小时的 LGHMF-MG 处理会抑制 hMSCs 的成骨作用。随着 LGHMF-MG 处理阶段的推迟，抑制强度逐渐降低。成骨诱导晚期（结束处理）的 6 小时 LGHMF-MG 处理对 hMSCs 的成骨作用没有明显影响。这些结果表明，LGHMF-MG 可能会影响成骨的起始。最后，讨论了 LGHMF-MG 抑制 hMSCs 成骨作用的可能机制。
