Centre for Nanoscience and Technology, Pondicherry University, Puducherry 605 014, India.
Centre for Nanoscience and Technology, Pondicherry University, Puducherry 605 014, India.
Int J Biol Macromol. 2017 Feb;95:928-937. doi: 10.1016/j.ijbiomac.2016.10.080. Epub 2016 Oct 29.
Copper ion (Cu) incorporated microporous chitosan-polyethylene glycol films were developed as a multipotent wound dressing material. The mechanical properties, swelling behavior and moisture permeability of hydrogel films with varying Cu concentration and the release of Cu from films were evaluated. The results revealed the enhanced mechanical stability of films with increasing Cu concentration without compromising moisture permeability and absorption capability of wound exudates. Microporous surface and swollen micropores in hydrated conditions were confirmed from morphological analysis. Degradation studies in lysozyme and HO demonstrated increasing stability of films with increasing Cu concentration up to 30days. Antibacterial tests revealed superiority in inhibiting biofilm formation in comparison to chitosan films. In vitro drug release, MTT assays and cell adhesion tests demonstrated the efficiency of Cu incorporated hydrogels in sustained drug release and antibacterial activity with excellent keratinocyte cell response.
铜离子（Cu）掺入微孔壳聚糖-聚乙二醇薄膜被开发为多效性伤口敷料材料。评估了具有不同 Cu 浓度的水凝胶薄膜的机械性能、溶胀行为和水分透过性以及 Cu 从薄膜中的释放。结果表明，随着 Cu 浓度的增加，薄膜的机械稳定性得到增强，同时不影响对伤口渗出物的水分透过性和吸收能力。从形态分析中证实了在水合条件下微孔表面和溶胀微孔的存在。在溶菌酶和 HO 中的降解研究表明，随着 Cu 浓度的增加，薄膜的稳定性逐渐提高，最高可达 30 天。抗菌试验表明，与壳聚糖薄膜相比，其在抑制生物膜形成方面具有优越性。体外药物释放、MTT 分析和细胞黏附试验表明，Cu 掺入水凝胶在持续药物释放和抗菌活性方面具有优异的角蛋白细胞反应效率。
