Institute of Environmental Biotechnology, University of Natural Resources and Life Sciences Vienna, Konrad Lorenz Strasse 20, 3430 Tulln, Austria.
Department of Biotechnology, University of Natural Resources and Life Sciences Vienna, Muthgasse 18, 1190 Vienna, Austria; Austrian Centre of Industrial Biotechnology GmbH, Petersgasse 14, 8010 Graz, Austria.
N Biotechnol. 2018 Jan 25;40(Pt B):236-244. doi: 10.1016/j.nbt.2017.09.004. Epub 2017 Sep 19.
A bioactive O-carboxymethyl chitosan (CMCS) hydrogel crosslinked with natural phenolics with potential application in wound dressings was synthesized using a laccase from Myceliophthora thermophila (MTL). The highest degree of cross-linking (49.7%) was achieved with catechol. All the phenolic-CMCS hydrogels synthesized showed excellent anti-oxidant properties with a free radical scavenging activity up to 4-fold higher than in the absence of the phenolics, as quantified by the di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH) assay. In addition, the hydrogels produced showed an anti-inflammatory effect as evidenced by the inhibition of enzymes [myeloperoxidase (MPO), matrix-metalloproteinase-1 (MMP-1) and human neutrophil elastase (HNE)] over-expressed in chronic wounds. Sinapyl-CMCS hydrogels showed an MMP-1 inhibition of 37%. Further, the phenolic-CMCS hydrogels did not affect the viability of the NIH 3T3 mouse fibroblast cell line and were also able to slowly release human fibroblast growth factor 2, reaching 48.3% over a period of 28days. This study thus shows the possibility of synthesizing multifunctional bioactive chitosan based hydrogels with anti-oxidant and anti-inflammatory properties using natural occurring phenolics as crosslinkers.
使用嗜热毁丝霉（Myceliophthora thermophila）来源的漆酶（laccase）合成了一种具有潜在应用于伤口敷料的生物活性 O-羧甲基壳聚糖（CMCS）水凝胶，该水凝胶与天然酚类物质交联。用儿茶酚实现了最高的交联度（49.7%）。所有合成的酚类-CMCS 水凝胶均表现出出色的抗氧化性能，其自由基清除活性比没有酚类物质时高出 4 倍，这是通过二（苯基）-（2,4,6-三硝基苯基）亚氨基脒（DPPH）测定法定量的。此外，水凝胶产生了抗炎作用，这一点可通过抑制慢性伤口中过度表达的酶[髓过氧化物酶（MPO）、基质金属蛋白酶-1（MMP-1）和人中性粒细胞弹性蛋白酶（HNE）]来证明。肉桂酰基-CMCS 水凝胶对 MMP-1 的抑制率为 37%。此外，酚类-CMCS 水凝胶不会影响 NIH 3T3 小鼠成纤维细胞系的活力，并且还能够缓慢释放人成纤维细胞生长因子 2，在 28 天内达到 48.3%。因此，这项研究表明，使用天然存在的酚类物质作为交联剂，有可能合成具有抗氧化和抗炎性能的多功能生物活性壳聚糖水凝胶。
