Institute of Organic Chemistry and Technology, Faculty of Chemical Technology, University of Pardubice, Studentská 95, CZ-532 10, Pardubice, Czech Republic.
Carbohydr Polym. 2013 Feb 15;92(2):1177-87. doi: 10.1016/j.carbpol.2012.08.098. Epub 2012 Sep 12.
Silver has been used since time immemorial in different chemical form to treat burns, wounds and several different infections caused by pathogenic bacteria, advancement of biological process of nanoparticles synthesis is evolving into a key area of nanotechnology. The current study deals with the green synthesis, characterization, and evaluation of the biological activity and cell viability of hyaluronan fibers with incorporated silver nanoparticles (HA-Ag NPs). Hyaluronan fiber was prepared by the dissolving of sodium hyaluronate (HA) in aqueous alkaline solution to prepare a transparent solution, which was used for the preparation of fibers by a wet-spinning technique. Consequently, hyaluronan fiber was used as capping and stabilizing agent for the preparation of fibers with silver nanoparticles. HA-Ag NPs were confirmed by transmission electron microscopy, dynamic light scattering, UV/VIS spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy, thermal analysis, nuclear magnetic resonance, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy. HA-Ag NPs showed high antibacterial activity of against Staphylococcus aureus and Escherichia coli. Cell viability tests indicated that hyaluronan, hyaluronan fibers and hyaluronan fibers with silver nanoparticles were non-toxic on the cell growth. Two different particles size of Ag NPs (10, 40 nm) had not any toxicity till the concentration limit. These tests were performed using mouse fibroblast cell line 3T3.
自古以来，银就以不同的化学形式被用于治疗烧伤、创伤和由致病细菌引起的几种不同感染，纳米粒子生物合成的进步正在成为纳米技术的一个关键领域。本研究涉及透明质酸纤维负载银纳米粒子（HA-Ag NPs）的绿色合成、表征以及生物活性和细胞活力的评价。通过将透明质酸钠（HA）溶解在碱性水溶液中制备透明溶液，然后通过湿法纺丝技术制备纤维，从而制备了透明质酸纤维。因此，透明质酸纤维可用作银纳米粒子纤维的封端和稳定剂。通过透射电子显微镜、动态光散射、紫外/可见光谱、扫描电子显微镜、能谱、热分析、核磁共振、傅里叶变换红外光谱和 X 射线光电子能谱对 HA-Ag NPs 进行了确认。HA-Ag NPs 对金黄色葡萄球菌和大肠杆菌表现出很高的抗菌活性。细胞活力试验表明，透明质酸、透明质酸纤维和负载银纳米粒子的透明质酸纤维对细胞生长无毒性。两种不同粒径的 Ag NPs（10、40nm）在浓度极限内没有任何毒性。这些测试是使用小鼠成纤维细胞系 3T3 进行的。
