Bio-Nano Electronics Research Centre, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama 350-8585, Japan.
Carbohydr Polym. 2013 Oct 15;98(1):108-15. doi: 10.1016/j.carbpol.2013.04.050. Epub 2013 May 17.
Mauran (MR), a highly polyanionic sulfated exopolysaccharide was extracted from moderately halophilic bacterium; Halomonas maura and characterized using X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy. Purified MR was evaluated for antioxidant defense mechanisms under in vitro conditions using L929, mouse fibroblast cell line and mice liver homogenate. It was demonstrated that MR could impart protective effect against oxidative stress in both cells and tissue up to a concentration of 500 μg, which is found to be safe under laboratory conditions. Various enzymatic and non-enzymatic parameters of antioxidant mechanisms were evaluated and concluded that MR has the tendency to maintain a balance of antioxidative enzymes with in the test systems studied. Also, hemocompatibility assay performed revealed that MR has a lesser hemolytic index and exhibited a prolonged clotting time, which shows both antihemolytic, and antithrombogenic nature respectively. Furthermore, absorption studies performed using fluorescent-labeled MR confirmed that MR accumulated within the cell cytoplasm neither induced cellular lysis nor affected the cell integrity.
从中度嗜盐菌；盐单胞菌中提取了高度带负电荷的硫酸化胞外多糖 Mauran (MR)，并使用 X 射线光电子能谱和傅里叶变换红外光谱对其进行了表征。使用 L929 小鼠成纤维细胞系和小鼠肝匀浆在体外条件下评估了纯化的 MR 对抗氧化防御机制的影响。结果表明，MR 可以在细胞和组织中提供抗氧化应激的保护作用，最高浓度可达 500μg，在实验室条件下被认为是安全的。评估了抗氧化机制的各种酶和非酶参数，并得出结论，MR 具有在研究的测试系统中维持抗氧化酶平衡的趋势。此外，进行的血液相容性试验表明，MR 的溶血指数较低，凝血时间延长，分别显示出抗溶血和抗血栓形成的特性。此外，使用荧光标记的 MR 进行的吸收研究证实，MR 积累在细胞质内既不诱导细胞裂解，也不影响细胞完整性。
