Institute of Animal Husbandry and Veterinary Medicine, Liaoning Medical University, No. 48 People's Street, Jinzhou, Liaoning 121001, PR China.
Carbohydr Polym. 2013 Feb 15;92(2):1901-7. doi: 10.1016/j.carbpol.2012.11.060. Epub 2012 Nov 29.
In this study, response surface methodology and central composite design based on single-factor experiments were applied to the optimization of the extraction parameters (extraction time, extraction temperature, and solid-liquid ratio) of polysaccharides from Kadsura marmorata fruits. The extraction temperature and solid-liquid ratio affected the extraction yield significantly. The optimal conditions included a solid-liquid ratio of 1:27 (mass/volume), an extraction time of 3h and an extraction temperature of 98 °C. Under these conditions, the maximal yield of crude Kadsura polysaccharide (KPS) was 2.7735±0.068%, which agreed with model predictions. Five major fractions, KPS I, KPS II, KPS III, KPS IV and KPS V, were obtained by diethylaminoethyl (DEAE) cellulose-52 column chromatography. The main fraction, KPS III-1, was isolated from KPS III by Sephadex G-100 column chromatography. Preliminary characterization of KPS III-1 by gas chromatography indicated that it was composed mainly of xylose and galacturonic acid.
在这项研究中，响应面法和基于单因素实验的中心复合设计被应用于优化五味子果实多糖的提取参数（提取时间、提取温度和固液比）。提取温度和固液比对提取产率有显著影响。最佳条件包括固液比为 1:27（质量/体积）、提取时间为 3 小时和提取温度为 98°C。在这些条件下，粗五味子多糖（KPS）的最大得率为 2.7735±0.068%，与模型预测相符。通过 DEAE 纤维素-52 柱层析得到了五个主要级分，即 KPS I、KPS II、KPS III、KPS IV 和 KPS V。从 KPS III 中通过 Sephadex G-100 柱层析分离出主要级分 KPS III-1。通过气相色谱初步表征 KPS III-1 表明，它主要由木糖和半乳糖醛酸组成。
