Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an, 710069, China.
Glycoconj J. 2018 Feb;35(1):107-117. doi: 10.1007/s10719-017-9806-3. Epub 2017 Dec 2.
Water-soluble polysaccharides from Umbilicaria tornata (UTP) were purified and preliminarily characterized. The antioxidant and antitumor activities of crude UTP and two purified fractions (UTP-1 and UTP-2) were evaluated using in vitro experiments. The results showed that the molecular weights of UTP-1 and UTP-2 were 84.86 and 28.66 kDa, respectively. Both UTP-1 and UTP-2 were composed of glucose and xylose, with their molar ratios being 1.3:0.9 and 0.9:4.6, respectively. In addition, crude UTP, UTP-1 and UTP-2 showed dose-dependent DPPH and hydroxyl radical scavenging and reducing activities. However, crude UTP exhibited stronger antioxidant activity than UTP-1 and UTP-2, particularly in terms of DPPH radicals. Crude UTP and the two purified fractions inhibited the growth of HeLa, HepG2, A375, MCF-7, SGC7901 and Caco2 cancer cells in vitro. Compared with UTP-1 and UTP-2, crude UTP presented significantly higher antitumor activity in vitro against HeLa and HepG2 cells (p < 0.05). These findings provide a scientific basis for the deeper exploration and resource development of U. tornata.
从裂褶菌（UTP）中纯化并初步表征了水溶性多糖。采用体外实验评价了粗 UTP 及两种纯化级分（UTP-1 和 UTP-2）的抗氧化和抗肿瘤活性。结果表明，UTP-1 和 UTP-2 的分子量分别为 84.86 和 28.66 kDa。UTP-1 和 UTP-2 均由葡萄糖和木糖组成，摩尔比分别为 1.3:0.9 和 0.9:4.6。此外，粗 UTP、UTP-1 和 UTP-2 均表现出剂量依赖性的 DPPH 和羟基自由基清除能力和还原能力。然而，粗 UTP 表现出比 UTP-1 和 UTP-2 更强的抗氧化活性，特别是在 DPPH 自由基方面。粗 UTP 及两种纯化级分均能抑制体外 HeLa、HepG2、A375、MCF-7、SGC7901 和 Caco2 癌细胞的生长。与 UTP-1 和 UTP-2 相比，粗 UTP 对 HeLa 和 HepG2 细胞的体外抗肿瘤活性显著更高（p < 0.05）。这些发现为进一步深入探索和资源开发裂褶菌提供了科学依据。
