Department of Polymer Science and Engineering, Chungnam National University, 220 Gung-dong, Yuseong-gu, Daejeon 305-764, Republic of Korea.
Carbohydr Polym. 2013 Feb 15;92(2):2267-75. doi: 10.1016/j.carbpol.2012.11.068. Epub 2012 Dec 7.
In this study, a new thermo-sensitive polymer, glycol chitin, was synthesized by controlled N-acetylation of glycol chitosan and evaluated as a thermogelling system. The physico-chemical properties of glycol chitins with different degrees of acetylation (DA) were investigated in terms of degradation, cytotoxicity, rheological properties, and in vitro and in vivo gel formation. Aqueous solutions of glycol chitins were flowable freely at room temperature but quickly became a durable gel at body temperature. Thermo-reversible sol-gel transition properties were observed with fast gelation kinetics. Glycol chitins with higher DA showed faster degradation in the presence of lysozyme. They exhibited no significant biological toxicity against human cell lines. An anti-cancer drug, doxorubicin, could be incorporated into the hydrogel by a simple mixing process and released in a sustained pattern over 13 days. Our findings suggest that glycol chitins could be useful as a new thermogelling biomaterial for drug delivery and injectable tissue engineering.
在这项研究中，通过对乙二醇壳聚糖进行可控 N-乙酰化，合成了一种新型温敏聚合物——乙二醇几丁质，并将其评估为一种温敏凝胶系统。根据降解、细胞毒性、流变学特性以及体外和体内凝胶形成情况，研究了不同乙酰化程度（DA）的乙二醇几丁质的物理化学性质。乙二醇几丁质的水溶液在室温下可自由流动，但在体温下迅速形成耐用凝胶。具有快速凝胶化动力学的温敏溶胶-凝胶转变特性。在溶菌酶存在下，具有较高 DA 的乙二醇几丁质表现出更快的降解。它们对人细胞系没有显著的生物毒性。通过简单的混合过程，可将抗癌药物阿霉素掺入水凝胶中，并在 13 天内持续释放。我们的研究结果表明，乙二醇几丁质可用作新型温敏生物材料，用于药物输送和可注射组织工程。
