Shamsipour Fereshteh, Zarnani Amir Hassan, Ghods Roya, Chamankhah Mahmood, Forouzesh Flora, Vafaei Sedigheh, Bayat Ali Ahmad, Akhondi Mohammad Mehdi, Ali Oghabian Mohammad, Jeddi-Tehrani Mahmood
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Avicenna J Med Biotechnol. 2009 Apr;1(1):27-31.
Conjugation of monoclonal antibodies to super paramagnetic nanoparticles is an effective method for cancer diagnosis and treatment. In this study the humanized anti her2/neu monoclonal antibody- Herceptin- was conjugated to super paramagnetic iron oxide (SPIO) nanoparticles using EDC method. The concentration of the conjugated antibodies was measured by Bradford assay. The antibody-nanoparticle conjugates were incubated with SKBR-3 and T47D human breast carcinoma cell lines and the presence of the conjugates on cell surface was confirmed by Prussian blue iron staining method. Conjugation of Herceptin to SPIO resulted in a precipitate-free conjugate containing 20µg antibody/mg SPIO. Prussian blue iron-staining of cells showed successful binding of the conjugates to the cell surfaces. Conjugation of monoclonal antibodies to SPIO may be a useful method for detection of tumor cells, especially by MRI techniques.
单克隆抗体与超顺磁性纳米颗粒的偶联是一种用于癌症诊断和治疗的有效方法。在本研究中，使用EDC方法将人源化抗her2/neu单克隆抗体——赫赛汀——与超顺磁性氧化铁（SPIO）纳米颗粒偶联。通过Bradford测定法测量偶联抗体的浓度。将抗体-纳米颗粒偶联物与SKBR-3和T47D人乳腺癌细胞系孵育，并通过普鲁士蓝铁染色法确认偶联物在细胞表面的存在。赫赛汀与SPIO的偶联产生了一种无沉淀的偶联物，其中含有20µg抗体/mg SPIO。细胞普鲁士蓝铁染色显示偶联物成功结合到细胞表面。单克隆抗体与SPIO的偶联可能是检测肿瘤细胞的一种有用方法，尤其是通过MRI技术。
