来自金星果（山榄科）的8个微卫星位点的分离与鉴定
Isolation and characterization of 8 microsatellite loci from Chrysophyllum gonocarpum (Sapotaceae).
作者信息
Rodrigues L A, Ruas E A, Signori N C, Costa B F, Farias-Missão D C L, Vieira B G, Conson A R O, Chaves C L, Delfini J, Ruas C F, Ruas P M
机构信息
Departamento de Agronomia, Centro de Ciências Agrárias, Universidade Estadual de Londrina, Londrina, PR, Brasil.
出版信息
Genet Mol Res. 2013 Feb 8;12(1):416-9. doi: 10.4238/2013.February.8.6.
Chrysophyllum gonocarpum is a tropical tree species that is very important in the recovery of heterogeneous forests and of degraded areas of permanent preservation. We identified microsatellite loci for C. gonocarpum to assess the genetic variability and the patterns of the population structure of the species. We isolated 8 microsatellite primers by using CT- and GT-enriched genomic libraries. We detected 2-4 alleles with 2.9 alleles per locus on average, by polymerase chain reaction. Test for cross-amplification showed that some loci were successfully amplified in 2 other Chrysophyllum species. The microsatellites can be used to assess the genetic diversity and population structure of C. gonocarpum. Some primer pairs can be amplified in C. marginatum and C. splendens.
金叶树是一种热带树种，在异质森林和永久性保护区退化区域的恢复中非常重要。我们鉴定了金叶树的微卫星位点，以评估该物种的遗传变异性和种群结构模式。我们通过使用富含CT和GT的基因组文库分离出8个微卫星引物。通过聚合酶链反应，我们检测到每个位点平均有2.9个等位基因，共2 - 4个等位基因。交叉扩增测试表明，一些位点在另外2种金叶树属物种中成功扩增。这些微卫星可用于评估金叶树的遗传多样性和种群结构。一些引物对可在边缘金叶树和壮丽金叶树中扩增。
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