塑造纳米颗粒动力学用于单细菌水平筛选和直接结合效率测量。
Sculpting nanoparticle dynamics for single-bacteria-level screening and direct binding-efficiency measurement.
作者信息
Shi Y Z, Xiong S, Zhang Y, Chin L K, Chen Y -Y, Zhang J B, Zhang T H, Ser W, Larrson A, Lim S H, Wu J H, Chen T N, Yang Z C, Hao Y L, Liedberg B, Yap P H, Wang K, Tsai D P, Qiu C-W, Liu A Q
机构信息
School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an, 710049, China.
School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore, 639798, Singapore.
出版信息
Nat Commun. 2018 Feb 26;9(1):815. doi: 10.1038/s41467-018-03156-5.
Particle trapping and binding in optical potential wells provide a versatile platform for various biomedical applications. However, implementation systems to study multi-particle contact interactions in an optical lattice remain rare. By configuring an optofluidic lattice, we demonstrate the precise control of particle interactions and functions such as controlling aggregation and multi-hopping. The mean residence time of a single particle is found considerably reduced from 7 s, as predicted by Kramer's theory, to 0.6 s, owing to the mechanical interactions among aggregated particles. The optofluidic lattice also enables single-bacteria-level screening of biological binding agents such as antibodies through particle-enabled bacteria hopping. The binding efficiency of antibodies could be determined directly, selectively, quantitatively and efficiently. This work enriches the fundamental mechanisms of particle kinetics and offers new possibilities for probing and utilising unprecedented biomolecule interactions at single-bacteria level.
光势阱中的粒子捕获和束缚为各种生物医学应用提供了一个多功能平台。然而，用于研究光学晶格中多粒子接触相互作用的实现系统仍然很少。通过配置一个光流体晶格，我们展示了对粒子相互作用和功能的精确控制，如控制聚集和多跳。由于聚集粒子之间的机械相互作用，单个粒子的平均停留时间从克莱默理论预测的7秒大幅缩短至0.6秒。光流体晶格还能够通过粒子驱动的细菌跳跃对抗体等生物结合剂进行单细菌水平的筛选。抗体的结合效率可以直接、选择性、定量和高效地确定。这项工作丰富了粒子动力学的基本机制，并为在单细菌水平探测和利用前所未有的生物分子相互作用提供了新的可能性。
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