Swenson J M, Facklam R R, Thornsberry C
Antimicrobics Investigation Branch, Centers for Disease Control, Atlanta, Georgia 30333.
Antimicrob Agents Chemother. 1990 Apr;34(4):543-9. doi: 10.1128/AAC.34.4.543.
Eighty-five strains of vancomycin-resistant gram-positive bacteria from three genera, Leuconostoc, Pediococcus, and Lactobacillus, were tested to determine susceptibility to 24 antimicrobial agents by broth microdilution and to 10 agents by disk diffusion. The MICs of vancomycin and teicoplanin ranged from 64 to greater than 512 micrograms/ml; however, the MICs of daptomycin, a new lipopeptide, were all less than or equal to 0.25 micrograms/ml. None of the organisms were resistant to imipenem, minocycline, chloramphenicol, gentamicin, or daptomycin. The MICs of penicillin were in the moderately susceptible range for all but three strains. Susceptibility to the other agents varied by genus and, in some cases, by species. When disk diffusion results were compared with MICs for drugs recommended for streptococci by the National Committee for Clinical Laboratory Standards, Villanova, Pa., few very major or major errors were obtained, but the number of minor errors was 19.3%. Therefore, we recommended that MIC testing be used instead of disk diffusion testing for these organisms.
对来自三个属（明串珠菌属、片球菌属和乳杆菌属）的85株耐万古霉素革兰氏阳性菌进行了检测，通过肉汤微量稀释法测定其对24种抗菌药物的敏感性，并通过纸片扩散法测定其对10种药物的敏感性。万古霉素和替考拉宁的最低抑菌浓度（MIC）范围为64至大于512微克/毫升；然而，新型脂肽达托霉素的MIC均小于或等于0.25微克/毫升。所有菌株对亚胺培南、米诺环素、氯霉素、庆大霉素或达托霉素均无耐药性。除三株菌株外，所有菌株对青霉素的MIC均处于中度敏感范围。对其他药物的敏感性因属而异，在某些情况下，也因种而异。当将纸片扩散法的结果与宾夕法尼亚州维拉诺瓦市美国国家临床实验室标准委员会推荐用于链球菌的药物的MIC进行比较时，很少出现极重大或重大误差，但 minor误差的数量为19.3%。因此，我们建议对这些菌株采用MIC检测而非纸片扩散法检测。
