Yamane N, Jones R N
Department of Pathology, University of Iowa College of Medicine, Iowa City.
Diagn Microbiol Infect Dis. 1991 Jul-Aug;14(4):337-45. doi: 10.1016/0732-8893(91)90025-b.
A total of 57 strains of ampicillin-resistant and -susceptible enterococci representing 10 species and 23 strains of vancomycin-resistant Gram-positive bacteria (Leuconostoc and Pediococcus) were tested to determine their susceptibility to 43 antimicrobial agents by the reference broth microdilution method. The drug MICs for the ampicillin-resistant enterococci were generally similar to those of ampicillin-susceptible strains, that is, highly resistant to cephalosporins, moderately susceptible or resistant to quinolones, and susceptible to "glycopeptides." Some investigational quinolones (PD127391, sparfloxacin, WIN57273), minocycline, and rifampin were highly active. Vancomycin-resistant strains were usually resistant to other "glycopeptides," for which correlation coefficients of MICs ranged from 0.881 to 0.978, except ramoplanin (MICs, 0.008-0.5 micrograms/ml). Most isolates resistant to vancomycin were susceptible to the newer quinolones, penicillins, aminoglycosides, clindamycin, and erythromycin, but highly resistant to cephalosporins. Discrepancies between the MICs and MBCs for glycopeptides were noted (greater than or equal to 8-fold, MBC50/MIC50), but not for ramoplanin. The vancomycin disk test was in 96.1% absolute agreement by identifying resistant strains without contributing false-susceptible, very major error.
采用参考肉汤微量稀释法，对总共57株代表10个种的氨苄西林耐药和敏感肠球菌以及23株耐万古霉素革兰氏阳性菌（明串珠菌属和片球菌属）进行检测，以确定它们对43种抗菌药物的敏感性。氨苄西林耐药肠球菌的药物最低抑菌浓度（MIC）通常与氨苄西林敏感菌株相似，即对头孢菌素高度耐药，对喹诺酮类药物中度敏感或耐药，对“糖肽类”敏感。一些研究性喹诺酮类药物（PD127391、司帕沙星、WIN57273）、米诺环素和利福平具有高活性。耐万古霉素菌株通常对其他“糖肽类”耐药，其MIC的相关系数在0.881至0.978之间，但雷莫拉宁除外（MIC为0.008 - 0.5微克/毫升）。大多数耐万古霉素分离株对新型喹诺酮类药物、青霉素类、氨基糖苷类、克林霉素和红霉素敏感，但对头孢菌素高度耐药。注意到糖肽类药物的MIC和最低杀菌浓度（MBC）之间存在差异（大于或等于8倍，MBC5/MIC5），但雷莫拉宁不存在这种差异。万古霉素纸片试验在鉴定耐药菌株时绝对符合率为96.1%，无假敏感的非常重大错误。
