Tuncay O C, Haselgrove J C, Frasca P, Piddington C, Shapiro I M
Department of Biochemistry, School of Dental Medicine, University of Pennsylvania, Philadelphia 19104.
Arch Oral Biol. 1990;35(2):113-8. doi: 10.1016/0003-9969(90)90172-7.
The oxidative state of periodontal tissues in situ is not known. Scanning microfluorimetry uses NADH fluorescence readings to provide a measure of a tissue's oxidative metabolic activity. Digitally recorded fluorescence signals were compiled to create a distribution map for this reduced pyridine nucleotide in the periodontal structures, which was then related to the morphology as seen by SEM. To distinguish between NADH fluorescence and intrinsic fluorescence of collagen, as well as to study the effect of oxygen deprivation, mitochondrial oxidative activity was inhibited by CO in some animals. Oxidative status and sensitivity to changes in cellular energy metabolism in the dento-alveolar complex were tissue specific; differences between tissues may play a part in the differential remodelling of the periodontium.
牙周组织在原位的氧化状态尚不清楚。扫描显微荧光测定法利用NADH荧光读数来衡量组织的氧化代谢活性。对数字记录的荧光信号进行汇编，以创建这种还原型吡啶核苷酸在牙周结构中的分布图，然后将其与扫描电子显微镜所见的形态学联系起来。为了区分NADH荧光和胶原蛋白的固有荧光，以及研究缺氧的影响，在一些动物中用一氧化碳抑制线粒体氧化活性。牙槽复合体中的氧化状态和对细胞能量代谢变化的敏感性具有组织特异性；组织之间的差异可能在牙周膜的差异性重塑中起作用。
