• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过 LC-SPE-NMR 鉴定链格孢菌真菌毒素及其对大豆（Glycine max）悬浮细胞培养的细胞毒性作用。

Identification of Alternaria alternata mycotoxins by LC-SPE-NMR and their cytotoxic effects to soybean (Glycine max) cell suspension culture.

机构信息

Laboratório de Bioquímica Micromolecular de Micro-organismos, Departamento de Química, Universidade Federal de São Carlos-UFSCar, Rodovia Washington Luiz, Km 235, 676 São Carlos-SP, Brazil.

出版信息

Molecules. 2013 Feb 26;18(3):2528-38. doi: 10.3390/molecules18032528.

DOI:10.3390/molecules18032528
PMID:23442929
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6270395/
Abstract

This present work describes the application of liquid chromatography-solid phase extraction-nuclear magnetic resonance spectroscopy to analyse Alternaria alternata crude extracts. Altenusin (1), alternariol (2), 3'-hydroxyalternariol monomethyl ether (3), and alternariol monomethyl ether (4), were separated and identified. High-resolution mass spectrometry confirmed the proposed structures. The cytotoxic effects of these compounds towards plants were determined using soybean (Glycine max) cell cultures as a model. EC(50) values which range from 0.11 (± 0.02) to 4.69 (± 0.47) μM showed the high cytotoxicity of these compounds.

摘要

本工作描述了液相色谱-固相萃取-核磁共振波谱法在分析链格孢菌粗提物中的应用。从Alternaria alternata 粗提物中分离并鉴定了altenusin（1）、 alternariol（2）、3'-羟基 alternariol 单甲醚（3）和 alternariol 单甲醚（4）。高分辨质谱证实了所提出的结构。这些化合物对植物的细胞毒性作用通过大豆（Glycine max）细胞培养作为模型来确定。EC（50）值范围从 0.11（±0.02）到 4.69（±0.47）μM 表明这些化合物具有很高的细胞毒性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4156/6270395/bd82c9c06f2a/molecules-18-02528-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4156/6270395/950e72c124c0/molecules-18-02528-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4156/6270395/10da076c2288/molecules-18-02528-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4156/6270395/bd82c9c06f2a/molecules-18-02528-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4156/6270395/950e72c124c0/molecules-18-02528-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4156/6270395/10da076c2288/molecules-18-02528-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4156/6270395/bd82c9c06f2a/molecules-18-02528-g003.jpg

相似文献

1
Identification of Alternaria alternata mycotoxins by LC-SPE-NMR and their cytotoxic effects to soybean (Glycine max) cell suspension culture.通过 LC-SPE-NMR 鉴定链格孢菌真菌毒素及其对大豆（Glycine max）悬浮细胞培养的细胞毒性作用。
Molecules. 2013 Feb 26;18(3):2528-38. doi: 10.3390/molecules18032528.
2
Degradation of the Alternaria mycotoxins alternariol, alternariol monomethyl ether, and altenuene upon bread baking.在面包烘焙过程中，交链孢霉毒素 alternariol、alternariol monomethyl ether 和 altenuene 的降解。
J Agric Food Chem. 2010 Sep 8;58(17):9622-30. doi: 10.1021/jf102156w.
3
Alternaria toxins in South African sunflower seeds: cooperative study.南非葵花籽中的交链孢菌毒素：合作研究。
Mycotoxin Res. 2017 Nov;33(4):309-321. doi: 10.1007/s12550-017-0290-1. Epub 2017 Jul 28.
4
Sulfoglucosides as Novel Modified Forms of the Mycotoxins Alternariol and Alternariol Monomethyl Ether.磺基葡萄糖苷作为霉菌毒素交链孢酚和交链孢酚单甲醚的新型修饰形式。
J Agric Food Chem. 2016 Nov 23;64(46):8892-8901. doi: 10.1021/acs.jafc.6b03120. Epub 2016 Nov 15.
5
Conjugation of the mycotoxins alternariol and alternariol monomethyl ether in tobacco suspension cells.在烟草悬浮细胞中，交链孢酚和交链孢酚单甲醚的缀合。
J Agric Food Chem. 2015 May 20;63(19):4728-36. doi: 10.1021/acs.jafc.5b00806. Epub 2015 May 7.
6
A new method for detection of five alternaria toxins in food matrices based on LC-APCI-MS.基于 LC-APCI-MS 的食品基质中五种交链孢毒素检测的新方法。
Food Chem. 2013 Sep 1;140(1-2):161-7. doi: 10.1016/j.foodchem.2012.12.065. Epub 2013 Feb 27.
7
Mycotoxins in ingredients of animal feeding stuffs: I. Determination of Alternaria mycotoxins in oilseed rape meal and sunflower seed meal.动物饲料原料中的霉菌毒素：I. 油菜籽粕和向日葵籽粕中链格孢属霉菌毒素的测定
Food Addit Contam. 1997 Apr;14(3):249-62. doi: 10.1080/02652039709374522.
8
Tracking emerging mycotoxins in food: development of an LC-MS/MS method for free and modified Alternaria toxins.追踪食品中的新型真菌毒素：游离和修饰的交链孢菌毒素的 LC-MS/MS 检测方法的建立。
Anal Bioanal Chem. 2018 Jul;410(18):4481-4494. doi: 10.1007/s00216-018-1105-8. Epub 2018 May 16.
9
[The detection of Alternaria mycotoxins in laboratory culture].[实验室培养物中链格孢霉菌毒素的检测]
Zentralbl Mikrobiol. 1992;147(3-4):197-206.
10
Analysis of alternariol and alternariol monomethyl ether in foodstuffs by molecularly imprinted solid-phase extraction and ultra-high-performance liquid chromatography tandem mass spectrometry.采用分子印迹固相萃取和超高效液相色谱串联质谱法分析食品中的 alternariol 和 alternariol monomethyl ether。
Food Chem. 2018 Mar 15;243:357-364. doi: 10.1016/j.foodchem.2017.09.125. Epub 2017 Sep 28.

引用本文的文献

1
Secondary metabolites of Alternaria alternate appraisal of their SARS-CoV-2 inhibitory and anti-inflammatory potentials.链格孢的次生代谢产物：对其抑制SARS-CoV-2和抗炎潜力的评估
PLoS One. 2025 Jan 24;20(1):e0313616. doi: 10.1371/journal.pone.0313616. eCollection 2025.
2
Natural resorcylic lactones derived from alternariol.源自链格孢酚的天然间苯二酚内酯。
Beilstein J Org Chem. 2024 Aug 30;20:2171-2207. doi: 10.3762/bjoc.20.187. eCollection 2024.
3
Bio-guided isolation of potential anti-inflammatory constituents of some endophytes isolated from the leaves of ground cherry (Physalis pruinosa L.) via ex-vivo and in-silico studies.

本文引用的文献

1
Greenhouse Evaluation of Products That Induce Host Resistance for Control of Scab, Melanose, and Alternaria Brown Spot of Citrus.诱导寄主抗性以防治柑橘疮痂病、黑星病和链格孢褐斑病产品的温室评估
Plant Dis. 2003 Jan;87(1):69-74. doi: 10.1094/PDIS.2003.87.1.69.
2
Benzopyranones from the endophytic fungus Hyalodendriella sp. Ponipodef12 and their bioactivities.苯并吡喃酮类化合物来源于内生真菌 Hyalodendriella sp. Ponipodef12 及其生物活性。
Molecules. 2012 Sep 25;17(10):11303-14. doi: 10.3390/molecules171011303.
3
Analysis of mycotoxins in barley using ultra high liquid chromatography high resolution mass spectrometry: comparison of efficiency and efficacy of different extraction procedures.
通过离体和计算机筛选研究，从酸浆果（Physalis pruinosa L.）叶片中分离的一些内生菌中生物导向分离潜在抗炎成分。
BMC Complement Med Ther. 2023 Apr 3;23(1):103. doi: 10.1186/s12906-023-03934-9.
4
Metabolite Formation by Fungal Pathogens of Potatoes ( L.) in the Presence of Bioprotective Agents.在生物保护剂存在的情况下，真菌病原体对马铃薯（L.）的代谢产物形成。
Int J Environ Res Public Health. 2023 Mar 22;20(6):5221. doi: 10.3390/ijerph20065221.
5
Anti-Inflammatory Effects Exerted by 14-Methoxyalternate C from Antarctic Fungal Strain sp. SF-7343 via the Regulation of NF-κB and JAK2/STAT3 in HaCaT Human Keratinocytes.南极真菌菌株 SF-7343 中的 14-甲氧基交替 C 通过调节 NF-κB 和 JAK2/STAT3 对 HaCaT 人角质形成细胞发挥抗炎作用。
Int J Mol Sci. 2022 Nov 24;23(23):14642. doi: 10.3390/ijms232314642.
6
Recent Advances in Phytotoxins: A Review of Their Occurrence, Structure, Bioactivity, and Biosynthesis.植物毒素的最新进展：关于其存在、结构、生物活性和生物合成的综述
J Fungi (Basel). 2022 Feb 9;8(2):168. doi: 10.3390/jof8020168.
7
Potent and Selective Inhibitors of Human Monoamine Oxidase A from an Endogenous Lichen Fungus .来自一种内生地衣真菌的人单胺氧化酶A的强效和选择性抑制剂
J Fungi (Basel). 2021 Oct 18;7(10):876. doi: 10.3390/jof7100876.
8
Critical Assessment of Mycotoxins in Beverages and Their Control Measures.饮料中真菌毒素的评估及其控制措施。
Toxins (Basel). 2021 Apr 30;13(5):323. doi: 10.3390/toxins13050323.
9
Antibiotics from Extremophilic Micromycetes.极端嗜微真菌产生的抗生素。
Russ J Bioorg Chem. 2020;46(6):903-971. doi: 10.1134/S1068162020060023. Epub 2020 Dec 28.
10
Identification of fungi associated with soybeans and effective seed disinfection treatments.与大豆相关的真菌鉴定及有效的种子消毒处理
Food Sci Nutr. 2019 Aug 22;7(10):3194-3205. doi: 10.1002/fsn3.1166. eCollection 2019 Oct.
采用超高效液相色谱-高分辨质谱法分析大麦中的真菌毒素：不同提取方法的效率和效果比较。
Talanta. 2012 Sep 15;99:712-9. doi: 10.1016/j.talanta.2012.07.010. Epub 2012 Jul 20.
4
Hyphenated chromatographic techniques for structural characterization and determination of masked mycotoxins.用于结构表征和掩蔽霉菌毒素测定的分段色谱技术。
J Chromatogr A. 2012 Sep 14;1255:145-52. doi: 10.1016/j.chroma.2012.02.057. Epub 2012 Mar 3.
5
On-line coupling of capillary electrochromatography, capillary electrophoresis, and capillary HPLC with nuclear magnetic resonance spectroscopy.毛细管电色谱、毛细管电泳和毛细管高效液相色谱与核磁共振波谱的在线联用。
Anal Chem. 1998 Aug 1;70(15):3280-5. doi: 10.1021/ac980063o.
6
Advances in structure elucidation of small molecules using mass spectrometry.利用质谱法进行小分子结构解析的进展
Bioanal Rev. 2010 Dec;2(1-4):23-60. doi: 10.1007/s12566-010-0015-9. Epub 2010 Aug 21.
7
Molecular, ecological and evolutionary approaches to understanding Alternaria diseases of citrus.从分子、生态和进化角度理解柑橘链格孢病害。
Mol Plant Pathol. 2003 Nov 1;4(6):435-46. doi: 10.1046/j.1364-3703.2003.00189.x.
8
Stable isotope dilution assays of alternariol and alternariol monomethyl ether in beverages.饮料中交链孢酚和交链孢酚单甲醚的稳定同位素稀释分析
J Agric Food Chem. 2009 Jun 24;57(12):5152-60. doi: 10.1021/jf900450w.
9
On-flow pulsed field gradient heteronuclear correlation spectrometry in off-line LC-SPE-NMR analysis of chemicals related to the chemical weapons convention.在与《化学武器公约》相关化学品的离线LC-SPE-NMR分析中采用在线脉冲场梯度异核相关光谱法
Anal Chem. 2009 Feb 1;81(3):1262-9. doi: 10.1021/ac802407t.
10
Cytotoxic metabolites from the fungal endophyte Alternaria sp. and their subsequent detection in its host plant Polygonum senegalense.来自真菌内生菌链格孢属的细胞毒性代谢产物及其随后在其宿主植物塞内加尔蓼中的检测。
J Nat Prod. 2008 Jun;71(6):972-80. doi: 10.1021/np070447m. Epub 2008 May 22.