Culhaoglu Belde, Erbek Selim S, Erbek Seyra, Hizal Evren
Department of Otorhinolaryngology, Baskent University, Ankara, Turkey.
Audiol Res. 2017 Jul 18;7(2):181. doi: 10.4081/audiores.2017.181.
Acoustic trauma is a common reason for hearing loss. Different agents are used to prevent the harmful effect of acoustic trauma on hearing. The aim of this study was to evaluate the potential preventive effect of (black cumin) oil in acoustic trauma. Our experimental study was conducted with 20 Sprague Downey female rats (mean age, 12 months; mean weight 250 g). All of the procedures were held under general anesthesia. Following otoscopic examinations, baseline-hearing thresholds were obtained using auditory brainstem responses (ABR). To create acoustic trauma, the rats were then exposed to white band noise of 4 kHz with an intensity level of 107 dB in a soundproof testing room. On Day 1 following acoustic trauma, hearing threshold measurements were repeated. The rats were divided into two groups as the study group (n: 10) and the controls (n: 10). 2 mL/kg/day of oil was given to the rats in the study group orally. On Day 4 following acoustic trauma, ABR measurements were repeated again. There was no difference between the baseline hearing thresholds of the rats before acoustic trauma (P>0.005). After the acoustic trauma, hearing thresholds were increased and there was no significant statistically difference between the hearing thresholds of the study and control groups (P=0.979). At the 4 day following acoustic trauma, hearing thresholds of the rats in control group were found to be higher than those in the study group (P=0.03). Our results suggest that oil has a protective effect against acoustic trauma in early period. This finding should be supported with additional experimental and clinical studies, especially to determine the optimal dose, duration and frequency of potential oil therapy.
声创伤是听力损失的常见原因。人们使用不同的药物来预防声创伤对听力的有害影响。本研究的目的是评估黑种草油在声创伤中的潜在预防作用。我们的实验研究使用了20只雌性斯普拉格-道利大鼠（平均年龄12个月；平均体重250克）。所有操作均在全身麻醉下进行。在耳镜检查后，使用听性脑干反应（ABR）获得基线听力阈值。为了造成声创伤，然后将大鼠置于隔音测试室中，暴露于强度为107分贝的4千赫白噪声中。在声创伤后的第1天，重复进行听力阈值测量。将大鼠分为研究组（n = 10）和对照组（n = 10）。给研究组的大鼠口服2毫升/千克/天的黑种草油。在声创伤后的第4天，再次重复进行ABR测量。声创伤前大鼠的基线听力阈值之间没有差异（P>0.005）。声创伤后，听力阈值升高，研究组和对照组的听力阈值之间没有显著的统计学差异（P = 0.979）。在声创伤后的第4天，发现对照组大鼠的听力阈值高于研究组（P = 0.03）。我们的结果表明，黑种草油在早期对声创伤具有保护作用。这一发现应通过更多的实验和临床研究来支持，特别是要确定黑种草油潜在治疗的最佳剂量、持续时间和频率。
