Cancer Risk Factor Branch, Cancer Prevention and Research Institute, Via il Vecchio 2, 50139 Florence, Italy.
Mutagenesis. 2013 May;28(3):315-21. doi: 10.1093/mutage/get005. Epub 2013 Feb 27.
Air quality is a primary environmental concern in highly industrialised areas, with potential health effects in children residing nearby. The Sarroch industrial estate in Cagliari province, Sardinia Island, Italy, hosts the world's largest power plant and the second largest European oil refinery and petrochemical park. This industrial estate produces a complex mixture of air pollutants, including benzene, heavy metals and polycyclic aromatic hydrocarbons. Thus, we conducted a cross-sectional study to evaluate the prevalence of malondialdehyde-deoxyguanosine adducts in the nasal epithelium of 75 representative children, aged 6-14 years, attending primary and secondary schools in Sarroch in comparison with 73 rural controls. Additionally, the levels of bulky DNA adducts were analysed in a subset of 62 study children. DNA damage was measured by (32)P-postlabelling methodologies. The air concentrations of benzene and ethyl benzene were measured in the school gardens of Sarroch and a rural village by diffusive samplers. Outdoor measurements were also performed in other Sarroch areas and in the proximity of the industrial estate. The outdoor levels of benzene and ethyl benzene were significantly higher in the school gardens of Sarroch than in the rural village. Higher concentrations were also found in other Sarroch areas and in the vicinity of the industrial park. The mean levels of malondialdehyde-deoxyguanosine adducts per 10(8) normal nucleotides ± standard error (SE) were 74.6±9.1 and 34.1±4.4 in the children from Sarroch and the rural village, respectively. The mean ratio was 2.53, 95% confidence interval (CI): 1.71-2.89, P < 0.001, versus rural controls. Similarly, the levels of bulky DNA adducts per 10(8) normal nucleotides ± SE were 2.9±0.4 and 1.6±0.2 in the schoolchildren from Sarroch and the rural village, respectively. The means ratio was 1.90, 95% CI: 1.25-2.89, P = 0.003 versus rural controls. Our study indicates that children residing near the industrial estate have a significant increment of DNA damage.
空气质量是高度工业化地区的主要环境问题之一，可能对附近居住的儿童健康产生影响。意大利撒丁岛卡利亚里省的萨拉赫工业园区是世界上最大的发电厂和第二大欧洲炼油厂和石化园区。该工业园区生产包括苯、重金属和多环芳烃在内的复杂空气污染物混合物。因此，我们进行了一项横断面研究，以评估在萨拉赫的 75 名代表性儿童（年龄 6-14 岁）的鼻上皮中丙二醛-脱氧鸟苷加合物的流行率，并与 73 名农村对照组进行比较。此外，还在 62 名研究儿童的亚组中分析了大量 DNA 加合物的水平。通过（32）P-后标记方法测量 DNA 损伤。通过扩散采样器在萨拉赫学校花园和农村村庄测量空气中的苯和乙苯浓度。还在其他萨拉赫地区和工业园区附近进行了室外测量。萨拉赫学校花园中的苯和乙苯室外浓度明显高于农村村庄。在其他萨拉赫地区和工业园区附近也发现了更高的浓度。每 10（8）个正常核苷酸的丙二醛-脱氧鸟苷加合物的平均值±标准误差（SE）分别为萨拉赫儿童的 74.6±9.1 和农村村庄儿童的 34.1±4.4。平均值比为 2.53，95%置信区间（CI）：1.71-2.89，P<0.001，与农村对照组相比。同样，萨拉赫和农村地区学童每 10（8）个正常核苷酸的大量 DNA 加合物的平均值±SE 分别为 2.9±0.4 和 1.6±0.2。平均值比为 1.90，95%CI：1.25-2.89，P=0.003，与农村对照组相比。我们的研究表明，居住在工业园区附近的儿童的 DNA 损伤明显增加。
