Govindarajan Parameswari, Schlewitz Gudrun, Schliefke Nathalie, Weisweiler David, Alt Volker, Thormann Ulrich, Lips Katrin Susanne, Wenisch Sabine, Langheinrich Alexander C, Zahner Daniel, Hemdan Nasr Y, Böcker Wolfgang, Schnettler Reinhard, Heiss Christian
Laboratory of Experimental Trauma Surgery, Justus-Liebig University, Department of Trauma Surgery, University Hospital of Giessen-Marburg, Giessen, Germany.
Med Sci Monit Basic Res. 2013 Feb 28;19:76-86. doi: 10.12659/MSMBR.883815.
Osteoporosis is a multi-factorial, chronic, skeletal disease highly prevalent in post-menopausal women and is influenced by hormonal and dietary factors. Because animal models are imperative for disease diagnostics, the present study establishes and evaluates enhanced osteoporosis obtained through combined ovariectomy and deficient diet by DEXA (dual-energy X-ray absorptiometry) for a prolonged time period.
MATERIAL/METHODS: Sprague-Dawley rats were randomly divided into sham (laparotomized) and OVX-diet (ovariectomized and fed with deficient diet) groups. Different skeletal sites were scanned by DEXA at the following time points: M0 (baseline), M12 (12 months post-surgery), and M14 (14 months post-surgery). Parameters analyzed included BMD (bone mineral density), BMC (bone mineral content), bone area, and fat (%). Regression analysis was performed to determine the interrelationships between BMC, BMD, and bone area from M0 to M14.
BMD and BMC were significantly lower in OVX-diet rats at M12 and M14 compared to sham rats. The Z-scores were below -5 in OVX-diet rats at M12, but still decreased at M14 in OVX-diet rats. Bone area and percent fat were significantly lower in OVX-diet rats at M14 compared to sham rats. The regression coefficients for BMD vs. bone area, BMC vs. bone area, and BMC vs. BMD of OVX-diet rats increased with time. This is explained by differential percent change in BMD, BMC, and bone area with respect to time and disease progression.
Combined ovariectomy and deficient diet in rats caused significant reduction of BMD, BMC, and bone area, with nearly 40% bone loss after 14 months, indicating the development of severe osteoporosis. An increasing regression coefficient of BMD vs. bone area with disease progression emphasizes bone area as an important parameter, along with BMD and BMC, for prediction of fracture risk.
骨质疏松症是一种多因素的慢性骨骼疾病，在绝经后女性中高度流行，受激素和饮食因素影响。由于动物模型对疾病诊断至关重要，本研究通过双能X线吸收法（DEXA）建立并评估了长期联合卵巢切除术和低营养饮食诱导的骨质疏松症模型。
材料/方法：将Sprague-Dawley大鼠随机分为假手术（剖腹）组和卵巢切除-低营养饮食（卵巢切除并给予低营养饮食）组。在以下时间点通过DEXA扫描不同骨骼部位：M0（基线）、M12（术后12个月）和M14（术后14个月）。分析的参数包括骨密度（BMD）、骨矿物质含量（BMC）、骨面积和脂肪百分比（%）。进行回归分析以确定从M0到M14期间BMC、BMD和骨面积之间的相互关系。
与假手术组大鼠相比，卵巢切除-低营养饮食组大鼠在M12和M14时的BMD和BMC显著降低。卵巢切除-低营养饮食组大鼠在M12时的Z值低于-5，但在M14时仍有所下降。与假手术组大鼠相比，卵巢切除-低营养饮食组大鼠在M14时的骨面积和脂肪百分比显著降低。卵巢切除-低营养饮食组大鼠的BMD与骨面积、BMC与骨面积以及BMC与BMD的回归系数随时间增加。这可以通过BMD、BMC和骨面积随时间和疾病进展的不同百分比变化来解释。
大鼠联合卵巢切除术和低营养饮食导致BMD、BMC和骨面积显著降低，14个月后骨丢失近40%，表明发生了严重骨质疏松症。随着疾病进展，BMD与骨面积的回归系数增加，强调骨面积与BMD和BMC一样，是预测骨折风险的重要参数。
