Athens Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA. Email:
Vet Pathol. 2013 Sep;50(5):926-9. doi: 10.1177/0300985813478209. Epub 2013 Feb 27.
An 18-month-old Charolais cow developed depression and drooling and was submitted for necropsy after euthanasia. The cow was 1 of 50 moved between 2 farms approximately 5 days before the onset of clinical disease. Gross findings included swollen and hemorrhagic areas of malacia in the frontal, temporal, and parietal lobes of the cerebral cortex. Microscopically there was a necrotizing meningoencephalitis with intranuclear astrocytic and neuronal eosinophilic viral inclusions in the frontal, temporal, and parietal cerebral cortex as well as in the basal nuclei and thalamus. The gross and microscopic findings were consistent with necrotizing meningoencephalitis caused by bovine herpesvirus (BHV-1 or BHV-5), and the diagnosis was confirmed by detection of bovine herpesviral antigen on fresh samples of brain via fluorescent antibody test using a monoclonal antibody against BHV-1 glycoprotein C.
一头 18 个月大的夏洛来牛出现抑郁和流口水症状，并在安乐死后被提交进行剖检。该牛是在临床疾病发作前约 5 天从 2 个农场转移的 50 头牛之一。大体病变包括大脑皮质额、颞和顶叶的软化和出血区域。显微镜下，有坏死性脑膜脑炎，在额、颞和顶叶大脑皮质以及基底核和丘脑，可见核内星形胶质细胞和神经元嗜酸性包涵体。大体和显微镜检查结果与由牛疱疹病毒（BHV-1 或 BHV-5）引起的坏死性脑膜脑炎一致，通过使用针对 BHV-1 糖蛋白 C 的单克隆抗体的荧光抗体试验，在新鲜脑组织样本中检测到牛疱疹病毒抗原，从而确诊了该疾病。
