分子方法能否检测结核分枝杆菌中 1%的异烟肼耐药性？
Can molecular methods detect 1% isoniazid resistance in Mycobacterium tuberculosis?
机构信息
International Reference Laboratory of Mycobacteriology, Statens Serum Institut, Copenhagen, Denmark.
出版信息
J Clin Microbiol. 2013 May;51(5):1596-9. doi: 10.1128/JCM.00472-13. Epub 2013 Feb 27.
Abstract
Patients may harbor both drug-susceptible and -resistant bacteria, representing heteroresistance. We studied mixtures of isoniazid-resistant and -susceptible Mycobacterium tuberculosis strains. Conventional drug susceptibility testing was the most sensitive method of detection, whereas the line probe assay and sequencing were not able to detect the clinically relevant 1% proportion of resistant bacteria.
摘要
患者可能同时携带对药物敏感和耐药的细菌，这代表了异质性耐药。我们研究了异烟肼耐药和敏感结核分枝杆菌菌株的混合物。传统的药物敏感性测试是最敏感的检测方法，而线探针分析和测序则无法检测到临床上相关的 1%耐药细菌比例。
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