Núcleo de Pesquisa e Pós-Graduação, Hospital Santa Casa de Belo Horizonte, Rua Domingos Vieira 590, Santa Efigênia, 30150-240, Belo Horizonte, MG, Brazil.
Diabetes Res Clin Pract. 2013 Apr;100(1):69-73. doi: 10.1016/j.diabres.2013.01.018. Epub 2013 Feb 27.
To evaluate the antioxidant capacity and concentrations of vascular endothelial growth factor (VEGF) and interleukin 6 (IL-6) in aqueous humor from patients with type 2 diabetes mellitus (T2DM) with or without retinopathy.
Aqueous humor was obtained during elective cataract surgery from T2DM patients with or without retinopathy and from healthy subjects. Reducing response was evaluated by MTT dye reduction and the generation of reactive oxygen species (ROS) was determined by chemiluminescence assay. Granulocytes were treated with phorbol dibutyrate (PDB)-stimulated. Cytokines were quantified by ELISA.
Antioxidant capacity of aqueous humor from patients with retinopathy was greater (P<0.05) than that of healthy controls or persons with diabetes without retinopathy. ROS production in PDB (protein kinase C activator)-stimulated granulocytes from T2DM patients with or without retinopathy was inhibited by autologous aqueous humor. Concentrations of VEGF and IL-6 were similar in aqueous humor from healthy controls and from patients without retinopathy, but lower (P<0.05) than those from T2DM patients with retinopathy. Plasma levels of VEGF and IL-6 were similar (P>0.05) in healthy controls and in T2DM patients with and without retinopathy.
Aqueous humor from T2DM patients with retinopathy exhibits elevated antioxidant activity with significant suppressive effect on ROS production and enhanced levels of locally secreted VEGF and IL-6 in comparison with T2DM patients without retinopathy. These results suggest an inflammatory profile in the absence of typical oxidative stress for T2DM patients with retinopathy, possibly resulting from the compensatory antioxidant response detected in the aqueous humor improving the ocular redox state.
评估 2 型糖尿病（T2DM）伴或不伴视网膜病变患者房水中的抗氧化能力和血管内皮生长因子（VEGF）、白细胞介素 6（IL-6）的浓度。
在选择性白内障手术期间从 T2DM 伴或不伴视网膜病变的患者和健康受试者中获得房水。通过 MTT 染料还原评估还原反应，通过化学发光测定法确定活性氧（ROS）的产生。用佛波醇二丁酸酯（PDB）刺激粒细胞。通过 ELISA 定量细胞因子。
伴视网膜病变的患者房水中的抗氧化能力（P<0.05）大于健康对照组或无糖尿病性视网膜病变的患者。PDB（蛋白激酶 C 激活剂）刺激的 T2DM 伴或不伴视网膜病变患者的粒细胞中 ROS 的产生被自身房水中的抗氧化剂抑制。健康对照组和无视网膜病变患者的房水中 VEGF 和 IL-6 的浓度相似，但低于（P<0.05）伴视网膜病变的 T2DM 患者。健康对照组和 T2DM 伴或不伴视网膜病变患者的血浆 VEGF 和 IL-6 水平相似（P>0.05）。
与无视网膜病变的 T2DM 患者相比，伴视网膜病变的 T2DM 患者房水中表现出较高的抗氧化活性，对 ROS 产生具有显著的抑制作用，并增强了局部分泌的 VEGF 和 IL-6 的水平。这些结果表明，在没有典型的氧化应激的情况下，伴视网膜病变的 T2DM 患者存在炎症特征，可能是由于房水中检测到的代偿性抗氧化反应改善了眼的氧化还原状态。
