Kanagawa Cancer Center Research Institute, Yokohama, Kanagawa, Japan.
Oral Surg Oral Med Oral Pathol Oral Radiol. 2013 Mar;115(3):385-92. doi: 10.1016/j.oooo.2012.12.013.
SIRT1, class III histone deacetylase, has been described to be up-regulated in various malignancies. However, the opposite results have been reported in other malignancies. Therefore, we investigated SIRT1 expression to clarify its biological behavior and identify its usefulness as a biomarker for head and neck squamous cell carcinoma (HNSCC).
SIRT1 expression was assessed by immunohistochemistry (IHC) conducted using samples from 437 consecutive HNSCC patients. Acetylated histone status and p53 expression were also examined.
IHC revealed 79.6% staining of SIRT1 in HNSCC, while almost all normal tissues showed positive staining. SIRT1 expression predominated in cases involving patients aged >65 years, lymph node negative, and early clinical stage cases. It was positively and statistically correlated with expression of acetylated histone H3K9 and H4K16, but not with p53. Multivariate analyses revealed that expression of SIRT1 was an independent and good indicator of prognosis.
SIRT1 是 III 类组蛋白去乙酰化酶，已被描述为在各种恶性肿瘤中上调。然而，在其他恶性肿瘤中则报道了相反的结果。因此，我们研究了 SIRT1 的表达，以阐明其生物学行为，并确定其作为头颈部鳞状细胞癌（HNSCC）的生物标志物的有用性。
使用来自 437 例连续 HNSCC 患者的样本，通过免疫组织化学（IHC）评估 SIRT1 的表达。还检查了乙酰化组蛋白状态和 p53 表达。
IHC 显示 HNSCC 中 SIRT1 的染色率为 79.6%，而几乎所有正常组织均显示阳性染色。SIRT1 的表达在年龄>65 岁、淋巴结阴性和早期临床分期的患者中占优势。它与乙酰化组蛋白 H3K9 和 H4K16 的表达呈正相关且具有统计学意义，但与 p53 无关。多变量分析显示，SIRT1 的表达是预后的独立且良好的指标。
