Umino Y, Kohama T, Sato T A, Sugiura A
Department of Measles Virus, National Institute of Health, Tokyo, Japan.
J Gen Virol. 1990 May;71 ( Pt 5):1199-203. doi: 10.1099/0022-1317-71-5-1199.
A series of monoclonal antibodies (MAbs) against the haemagglutinin-neuraminidase (HN) and fusion (F) glycoproteins and the matrix (M) protein of Newcastle disease virus (NDV) were tested for protective effects in passive immunization of newborn chickens against challenge with a virulent heterologous strain of NDV (Italien). MAbs with high virus-neutralizing activity directed to one antigenic site of the HN protein delayed virus growth and significantly prolonged survival time, but all chickens eventually succumbed to infection. MAbs directed to two antigenic sites of the F protein completely suppressed virus growth and prevented death of chickens, although the neutralizing activities of these anti-F MAbs were lower than those of the above anti-HN MAbs. Combined administration of the anti-HN and anti-F MAbs had a synergistic protective effect, but no protective effects were shown by MAbs against the M protein.
检测了一系列针对新城疫病毒（NDV）血凝素神经氨酸酶（HN）、融合（F）糖蛋白和基质（M）蛋白的单克隆抗体（MAb），用于新生鸡被动免疫，以抵抗强毒异源NDV毒株（意大利株）的攻击。针对HN蛋白一个抗原位点具有高病毒中和活性的单克隆抗体可延迟病毒生长并显著延长存活时间，但所有鸡最终都死于感染。针对F蛋白两个抗原位点的单克隆抗体完全抑制了病毒生长并防止鸡死亡，尽管这些抗F单克隆抗体的中和活性低于上述抗HN单克隆抗体。联合使用抗HN和抗F单克隆抗体具有协同保护作用，但针对M蛋白的单克隆抗体未显示出保护作用。
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