Department of Andrology, Liuzhou Hospital of Traditional Chinese Medicine, Liuzhou, 545001, China.
World J Urol. 2013 Dec;31(6):1395-401. doi: 10.1007/s00345-013-1023-5. Epub 2013 Mar 2.
Idiopathic asthenozoospermia is considered as one of the causes of male infertility and characterized by reduced sperm motility. For a better determination of pathogenic mechanism of asthenozoospermia, the exploration of differentially expressed proteins in normal sperm motility and idiopathic asthenozoospermia was conducted in our study.
Sperm proteins were extracted and isolated by two-dimensional electrophoresis. All significantly changed protein spots were picked up from 2D gels and identified by tandem mass spectrometry. Sixteen of the thirty-three total differentially expressed protein spots were successfully identified by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry.
Sixteen proteins identified belonged to 15 unique protein groups. GRP78, lactoferrin, SPANXB, PGK2, flagellin, DJ-1, XPA binding protein 2, CAB2, GPX4, and GAPDH were the first to be identified as differentially expressed proteins in idiopathic asthenospermia patients. Meanwhile, the analysis of quantitative RT-PCR was carried out to compare the protein levels, and the results indicated that the expression levels of the gene and protein were not entirely consistent.
These experimental results expand the scope of the protein database, generating targets for further investigation of the pathogenic mechanism of idiopathic asthenozoospermia.
特发性弱精症被认为是男性不育的原因之一，其特征是精子运动能力下降。为了更好地确定弱精症的发病机制，我们对正常精子运动和特发性弱精症中的差异表达蛋白进行了探索。
通过二维电泳提取和分离精子蛋白。从 2D 凝胶中提取并鉴定了所有差异显著的蛋白点，采用基质辅助激光解吸电离飞行时间串联质谱法。
共鉴定出 33 个差异表达蛋白点中的 16 个，通过基质辅助激光解吸电离飞行时间串联质谱法鉴定出 16 种蛋白质，属于 15 种独特的蛋白质组。GRP78、乳铁蛋白、SPANXB、PGK2、鞭毛蛋白、DJ-1、XPA 结合蛋白 2、CAB2、GPX4 和 GAPDH 是首次被鉴定为特发性弱精症患者差异表达蛋白。同时，我们进行了定量 RT-PCR 分析以比较蛋白水平，结果表明基因和蛋白的表达水平并不完全一致。
这些实验结果扩展了蛋白质数据库的范围，为进一步研究特发性弱精症的发病机制提供了新的靶点。
