Nagy K, Jan A, Bánhegyi D, Várnai F
MTA Izotópkutató Intézete, Budapest.
Orv Hetil. 1990 Apr 29;131(17):895-8.
Human immunodeficiency virus infected persons are usually identified by indirect methods, detecting viral-specific antibodies. Helping the early diagnosis of HIV infection there is a need to detect virus or viral specific antigens directly. Virus isolation have been attempted from separated lymphocytes of HIV infected five homosexual male patients and one transfusion recipient. Three patients had AIDS, three belonged to the ARC group at the time of the examination. In 3 out of 6 lymphocyte cultures cocultivated with normal donor lymphocytes, virus antigens and virus replications had been detected within ten days of culture. The amount of HIV antigen p24 ranged between 0.5----2.0 ng/ml during the first two weeks of cocultivation. Permanent human lymphoid and monocyte/macrophage cell lines have been infected by the viruses isolated from the primer lymphocyte cocultures. Productive infection could be initiated in Jurkat tat-III and U937 cells, while infection of Jurkat and HUT 78 cell lines was transient. Results indicate, that HIVs could be isolated from infected Hungarian patients with various stages of AIDS and one isolate--termed HIV-1FB918--actively replicates in human permanent cell lines.
人类免疫缺陷病毒感染者通常通过检测病毒特异性抗体的间接方法来识别。为了有助于早期诊断HIV感染，需要直接检测病毒或病毒特异性抗原。已尝试从5名同性恋男性患者和1名输血接受者的分离淋巴细胞中分离病毒，这些患者均感染了HIV。3名患者患有艾滋病，3名在检查时属于艾滋病相关综合征（ARC）组。在与正常供体淋巴细胞共培养的6个淋巴细胞培养物中，有3个在培养10天内检测到病毒抗原和病毒复制。在共培养的前两周，HIV抗原p24的量在0.5----2.0 ng/ml之间。从原始淋巴细胞共培养物中分离出的病毒已感染了永久性人类淋巴细胞和单核细胞/巨噬细胞系。在Jurkat tat-III和U937细胞中可引发生产性感染，而Jurkat和HUT 78细胞系的感染是短暂的。结果表明，HIV可从患有不同阶段艾滋病的匈牙利感染患者中分离出来，并且一种名为HIV-1FB918的分离株在人类永久性细胞系中能活跃复制。
